乌司他丁对新生大鼠脓毒症肺损伤缓解作用的研究OA北大核心CSTPCD

Objective To investigate the molecular mechanism of ulinastatin in reducing lung injury in neonatal sepsis rats.Methods Sepsis-induced lung injury patients(sepsis lung injury group)and healthy newborns born during the same period(control group)were selected as research subjects,and venous blood from newborns of each group was collected for use.Newborn SD rats were randomly divided into blank control group(given an equal volume of 0.9％NaCl),model group(4 mg·kg-1 lipopolysaccharide),and ulinastatin group(4 mg·kg-1 lipopolysaccharide+5 × 104 U·kg-1 ulinastatin),with 12 rats in each group.Survival of the rats within 24 hours of injection was observed,and after 24 hours,blood was collected from the heart,and lung tissues were collected.Real-time fluorescent quantitative polymerase chain reaction was used to detect the expression of CRNDE and miR-29a in serum and lung tissue.Enzyme-linked immunosorbent assay was used to detect the expression of inflammatory factors such as interleukin 6(IL-6).Dual-luciferase reporter gene assay was used to verify the interaction between CRNDE and miR-29a.Kit methods were used to detect reactive oxygen species(ROS)and other oxidative stress indicators.Western blotting was used to detect the expression of heme oxygenase 1(HO-1).In situ fluorescence hybridization was used to detect the expression of CRNDE and miR-29a.Results The serum CRNDE expression levels in control group and sepsis lung injury group were 1.00±0.16 and 0.41±0.09,respectively;the expression levels of miR-29a were 1.00±0.14 and 1.83±0.25,respectively;IL-6 levels were(4.13±0.86)and(12.61±2.57)ng·mL-1,respectively.Compared with the control group,the above indexes in the sepsis lung injury group were statistically significant(all P＜0.05).The lung injury scores of blank control group,model group and ulinastatin group were 0.62±0.24,4.96±1.28,2.13±0.86,respectively;ROS levels were(0.93±0.15),(2.61±0.35),(1.58±0.23)U·mg-1,respectively;the protein levels of HO-1 were 1.00±0.17,2.19±0.31,1.17±0.16,respectively;IL-6 levels were(46.15±7.62),(186.77±21.30),(113.46±14.68)pg·mL-1,respectively;the expression levels of CRNDE were 1.00±0.12,0.41±0.06,0.82±0.13,respectively;the expression levels of miR-29a were 1.00±0.14,2.38±0.21 and 1.62±0.19,respectively.The above indexes were compared between blank control group and sepsis lung injury group,model group and blank control group,ulinastatin group and model group,and the differences were statistically significant(all P＜0.05).Conclusion Ulinastatin may alleviate lung injury in neonatal sepsis through IncRNA CRNDE/miR-29a signaling pathway.