中国临床药理学杂志2024，Vol.40Issue(15)：2217-2221,5.DOI:10.13699/j.cnki.1001-6821.2024.15.014
黄芪提取物调控Nrf2/ARE信号通路对糖尿病肾病大鼠氧化应激的影响
Effects of Astragalus extract regulating Nrf2/ARE signaling pathway on oxidative stress in diabetic nephropathy rats
摘要
Abstract
Objective To investigate the effect of Astragalus extract on oxidative stress in diabetic nephropathy rats by regulating nuclear factor E2-related factor 2(Nrf2)/antioxidant response element(ARE)signaling pathway.Methods The diabetic rat model was constructed by high-fat diet combined with intraperitoneal injection of streptozotocin,and was randomly divided into model group,experimental-L group,experimental-H group,experimental-H+ML385 group,with 12 rats in each group,and 12 rats were selected as blank group.Rats in blank group and model group were intragastric with equal volume of normal saline;rats in experimental-L,-H groups were intragastric with 50 mg·kg-1 astragalus extract and 100 mg·kg-1 Astragalus extract,respectively;rats in experimental-H+ML385 group were intragastric with 100 mg·kg-1 Astragalus extract and intraperitoneally injected with 20 mg·kg-1 ML385 once a day.Eight weeks in a row.The content of oxidative stress-related indexes in rat renal tissues was detected,the expression level of reactive oxygen species was detected by dihydroethidine staining,and the protein expression level of quinone oxidoreductase 1(NQO1),heme oxygenase 1(HO-1)and nuclear Nrf2 in rat renal tissues was detected by Western blot.Results Superoxide dismutase in blank group,model group,experimental-H group and experimental-H+ML385 group were(163.89±20.28),(71.35±12.72),(132.11±19.29)and(73.04±13.28)U·mg-1,respectively;glutathione peroxidase were(12.82±1.57),(4.91±1.18),(8.54±1.09),(5.10±1.43)U·mg-1,respectively;reactive oxygen species were 0.02±0.01,0.09±0.01,0.05±0.01 and 0.08±0.01,respectively;nuclear Nrf2 values were 0.63±0.09,0.28±0.06,0.60±0.08,0.32±0.05,respectively;the NQO1 values were 0.58±0.11,0.27±0.07,0.63±0.12 and 0.31±0.08,respectively;the HO-1 values were 0.53±0.08,0.23±0.06,0.59±0.09 and 0.28±0.05,respectively.Compared with the model group,the above indexes in the experimental-H group were statistically significant(all P＜0.05).The above indexes of the experimental-H+ML385 group were statistically significant compared with the experimental-H group(all P＜0.05).Conclusion Astragalus extract can alleviate oxidative stress damage in diabetic nephropathy rats,and the mechanism may be achieved by regulating Nrf2/ARE signaling pathway.关键词
黄芪提取物/糖尿病肾病/核因子E2相关因子2/抗氧化反应元件/氧化应激Key words
Astragalus extract/diabetic nephropathy/nuclear factor E2-related factor 2/antioxidant response element/oxidative stress分类
医药卫生引用本文复制引用
吴乙偲,谭菲,易露露,宋长军..黄芪提取物调控Nrf2/ARE信号通路对糖尿病肾病大鼠氧化应激的影响[J].中国临床药理学杂志,2024,40(15):2217-2221,5.基金项目
江西省卫生健康委科技计划基金资助项目(202311870) （202311870）
江西省中医药管理局科技计划基金资助项目(2022A255) （2022A255）
