中国临床药理学杂志2024，Vol.40Issue(15)：2227-2230,4.DOI:10.13699/j.cnki.1001-6821.2024.15.016
脑源性神经营养因子在谷氨酸钠诱导的中枢性肥胖小鼠海马组织中表达的研究
Expression of brain-derived neurotrophic factor in hippocampal tissue of central obese mice induced by sodium glutamate
摘要
Abstract
Objective To explore whether the cognitive function of central obese mice is decreased by affecting the expression of brain-derived neurotrophic factor(BDNF)in hippocampus.Methods Healthy mice at the neonatal stage were divided into normal group and model group at random.To obtain the obese models,model group mice were injected at cervical subcutaneous with 10％L-monosodium glutamate(MSG;3 mg·g-1·d-1)for 5 days.The normal group was injected with the same dose of 0.9％NaCl.In addition,mice were removed according to the requirements.Finally,we got 8 mice in each group.The following parameters were compared:body weight,Lee's index and levels of the serum lipid.The BDNF expression levels in hippocampal tissue were measured using western blotting.Results At the 8th weekend,the body weight of the model and normal groups was(49.01±2.47)and(41.27±3.28)g;the Lee's indexes were(357.14±9.24)and(330.15±7.37)g1/3·cm-1;triglyceride levels were(1.37±0.52)and(0.73±0.31)mmol·L-1;total cholesterol levels were(2.98±0.18)and(1.98±0.30)mmol·L-1;low-density lipoprotein levels were(0.31±0.03)and(0.24±0.02)mmol·L-1;high-density lipoprotein levels were(2.70±0.15)and(1.98±0.40)mmol·L-1;the differences were statistically significant(P＜0.05,P＜0.01),which were consistent with the characteristics of the central obesity model.The BDNF protein expression levels in the hippocampus of the model and normal groups were 6.02 x 104±626.53 and 7.04 x 104±1 440.81,which has statistically significant(P＜0.01).Conclusion The cognitive function of central obese mice may be decreased by down-regulating the expression of BDNF in hippocampus.关键词
脑源性神经营养因子/谷氨酸钠/中枢性肥胖/海马/认知功能Key words
brain-derived neurotrophic factor/monosodium glutamate/central obesity/hippocampus/cognitive function分类
医药卫生引用本文复制引用
曹鹏娟,唐嘉媛,杨美子,李园园,黄立婷,蒙雯雯,金勇君,莫建勋..脑源性神经营养因子在谷氨酸钠诱导的中枢性肥胖小鼠海马组织中表达的研究[J].中国临床药理学杂志,2024,40(15):2227-2230,4.基金项目
广西壮族自治区卫生健康委员会自筹经费科研课题资助项目(Z-C20221045) （Z-C20221045）
桂林市科学研究与技术开发计划基金资助项目(20220139-5-1) （20220139-5-1）
