中国临床药理学杂志2024，Vol.40Issue(15)：2231-2235,5.DOI:10.13699/j.cnki.1001-6821.2024.15.017
炙红芪对脾气虚大鼠肠黏膜屏障保护的研究
Study of honey-processed Hedysari Radix on the protection of intestinal mucosal barrier in rats with spleen deficiency
摘要
Abstract
Objective To explore the protective mechanism of honey-processed Hedysari Radix in regulating intestinal mucosal injury in rats with spleen qi deficiency.Methods The three-factor composite modeling method of bitter cold diarrhea,overwork and hunger and satiety disorder was used to construct a spleen qi deficiency model rats.After the model was successfully made,they were randomly divided into model group,honey-processed Hedysari Radix group and probiotic group,with 15 animals in each group.Another 15 normal rats were taken as the blank group.The honey-processed Hedysari Radix group was given 12.6 g·kg-1 water decoction of honey-processed Hedysari Radix by gavage,the probiotics group was given Bifidobacterium Lactobacillus triple viable tablets suspension at a dose of 0.625 g·kg-1,and the blank group and the model group were given the same dose of distilled water.The rats in the four groups were administered once a day for 15 days.Enzyme-linked immunosorbent assay was used to detect diamine oxidase(DAO)in serum,D-lactic acid(D-LA),secretory immunoglobulin A factor,and Western blotting was used to detect the expression levels of AMP-activated protein kinase(AMPK),zonula occludens-1(ZO-1)and occludin in colon tissues.Results The serum levels of DAO in the blank group,model group,honey-processed Hedysari Radix group and probiotic group were(138.93±9.78),(187.95±12.90),(147.21±6.92)and(166.47±3.37)pg·mL-1;the contents of D-LA were(892.23±49.17),(1 099.84±137.64),(956.56±86.04)and(989.61±51.75)μg·L-1;the contents of SIgA in colon tissues were(14.04±1.42),(11.47±2.39),(11.84±1.49)and(12.93±1.65)μg·mL-1;the relative expression levels of ZO-1 protein in colon tissues were 1.18±0.11,0.42±0.04,0.77±0.05 and 0.95±0.07;the relative expression levels of occludin protein were 1.35±0.31,0.61±0.17,1.19±0.19 and 0.88±0.13;the relative expression levels of AMPK protein were 0.91±0.02,0.35±0.09,0.74±0.08 and 0.59±0.11.Compared with the model group,there were significant differences in the serum content of DAO and D-LA,SIgA content in colon,and the content of ZO-1,occludin and AMPK protein in the honey-processed Hedysari Radix group(P＜0.01,P＜0.05).Conclusion Honey-processed Hedysari Radix can enhance the protective effect on the intestinal mucosa of rats with spleen qi deficiency by regulating the expression of related inflammatory cytokines,intestinal mucosal upper cell enzymes and tight junction proteins in rats with spleen qi deficiency.关键词
炙红芪/脾气虚证/肠黏膜屏障/保护作用Key words
honey-processed Hedysari Radix/spleen qi deficiency syndrome/intestinal mucosal barrier/protective effect分类
医药卫生引用本文复制引用
王毛毛,李越峰,宋沁洁,王哲,马定财,张育贵,刘婷,张转红,高飞云,王彦钧..炙红芪对脾气虚大鼠肠黏膜屏障保护的研究[J].中国临床药理学杂志,2024,40(15):2231-2235,5.基金项目
国家自然科学基金资助项目(81960713,82160750) （81960713,82160750）
甘肃省教育厅产业支撑计划基金资助项目(2021CYZC-21) （2021CYZC-21）
甘肃省科技计划(创新基地与人才计划)基础研究创新群体基金资助项目(21JR7RA569) （创新基地与人才计划）
