中国输血杂志2024，Vol.37Issue(8)：933-939,7.DOI:10.13303/j.cjbt.issn.1004-549x.2024.08.015
献血者HBVs基因变异的生物信息学分析
Bioinformatics analysis of HBV s gene mutation in blood donor
摘要
Abstract
Objective To analyze the infectious indicators,HBV s gene mutations and changes in bioinformatics char-acteristics of HBV DNA+/HBsAg-blood donor.Methods A sample of HBV DNA+/HBsAg-was screened by PCR meth-od,and HBsAg/HBsAb/HBeAg/HBeAb/HBcAb of HBV in the sample were detected by ELISA and chemiluminescence methods.The mutation of the HBVs gene fragment in the sample was sequenced and analyzed,and the bioinformatics analy-sis was performed using analysis software.Results The sample showed HBV DNA+,HBsAg-,HBsAb+,HBeAg+,HBe-Ab-and HBcAb+,with an amino acid unit point mutation(P151L)occurred in the HBV s gene,and the spatial structure changed.Conclusion The spatial structural changes in the gene sequence of HBVs may be the reason for the appearance of serological HBsAg-/HBsAb+/HBV DNA+in the test results.关键词
HBV/OBI/s基因/献血者/生物信息学Key words
HBV/OBI/s gene/blood donor/bioinformatics分类
医药卫生引用本文复制引用
范加诚,李青,陈秀丽,刘世香,陈烨..献血者HBVs基因变异的生物信息学分析[J].中国输血杂志,2024,37(8):933-939,7.基金项目
泰安市科技创新发展项目(2023NS450) （2023NS450）
