中国兽医科学2024，Vol.54Issue(8)：995-1001,7.DOI:10.16656/j.issn.1673-4696.2024.0132
非洲猪瘟病毒毒力因子MGF360-4L靶向降解RIPK3促进病毒复制的研究
Virulence factor MGF360-4L of African swine fever virus facilitates viral replication by degrading RIPK3
摘要
Abstract
In the present study,the effects of MGF360-4L on cell viability were first determined by CellTiter-Glo kit.The effects of MGF360-4L and its mutants on the expression of key adaptors of necrosis pathway were detected by Western-blot.MGF360-4L-deficient ASFV(ASFV-ΔMGF360-4L)was constructed by homologous recombination technique.Real-time quantitative fluorescent PCR(RT-qPCR)was used to analyze the replication difference between wild-type ASFV(ASFV-WT)and ASFV-ΔMGF360-4L.The effects of MGF360-4L deletion on ASFV-induced necrosis were detected by Western blot.These results indicated that MGF360-4L contained a RHIM and degraded RIPK3 via the lysosomal pathway,thereby inhibiting the activation of necrosis signal.MGF360-4L promoted the degradation of RIPK3 in a RHIM domain-dependent manner.ASFV-ΔMGF360-4L had a lower replication level than that of ASFV-WT,and the deficient strain induced higher levels of necrosis.Altogether,our study suggests that ASFV virulence factor MGF360-4L degrades RIPK3 relied on its RHIM motif and promotes viral replication,which provides scientific data for the elucidation of ASFV pathogenesis.关键词
非洲猪瘟病毒/细胞坏死/RHIM/RIPK3/MGF360-4LKey words
African swine fever virus/necroptosis/RHIM/RIPK3/MGF360-4L分类
农业科技引用本文复制引用
曹雪静,郑海学,万博,李伟伟,范许许,朱兆宇,裴丹诗,王一卓,张继燕,何路,李熙忠,任青峰..非洲猪瘟病毒毒力因子MGF360-4L靶向降解RIPK3促进病毒复制的研究[J].中国兽医科学,2024,54(8):995-1001,7.基金项目
国家重点研发计划项目(2021YFD1801300,2021YFD1800300) （2021YFD1801300,2021YFD1800300）
家畜重要疫病感染与免疫防控的基础理论研究项目(22ZD6NA001) （22ZD6NA001）
中央高校基本科研业务费专项资金资助(学科交叉创新团队建设项目)(lzujbky-2022-ct02) （学科交叉创新团队建设项目）
国家自然科学基金青年项目(32202781,32302857) （32202781,32302857）
甘肃省青年科技基金计划项目(22JR5RA034) （22JR5RA034）
中国博士后科学基金项目(2023M743830) （2023M743830）
国家生猪产业技术体系项目(CARS-35,CARS-39-13) （CARS-35,CARS-39-13）
国家生猪技术创新中心先导科技项目(NCTIP-XD/C03) （NCTIP-XD/C03）
中国农业科学院创新计划项目(CAAS-CSLPDCP-2023002,CAAS-ASTIP-2023-LVRI) （CAAS-CSLPDCP-2023002,CAAS-ASTIP-2023-LVRI）
"十四五"广东省农业科技创新十大主攻方向"揭榜挂帅"项目(2023SDZG02) （2023SDZG02）
兰州兽医研究所基本科研业务项目(1610312021009) （1610312021009）
家畜重要疫病诊断研制与产业化项目(22ZD6NA012) （22ZD6NA012）
