中国兽医科学2024，Vol.54Issue(8)：1019-1026,8.DOI:10.16656/j.issn.1673-4696.2024.0127
传染性支气管炎病毒诱导Vero细胞发生自噬促进病毒的复制
IBV promotes self-replication by inducing autophagy in Vero cells
摘要
Abstract
In this study,to investigate whether avian Infectious bronchitis virus(IBV)can induce autophagy in cells and its effect on viral replication,DF-1 and Vero cells were inoculated with cell-adapted strain CEA of IBV.Then the expression and distribution of key proteins of autophagy path-way,LC3 and P62,and the formation of autophagosomes were detected by Western-blot,transmission elec-tron microscopy and confocal laser microscopy,respectively.The effect of autophagy on IBV replication was further evaluated by the treatment of autophagy inhibitor and inducer.The results showed that IBV did not induce LC3 transformation in DF-1 cells,but could increase levels of LC3-Ⅱ and P62 in Vero cells,as well as the formation of autophagosomes in Vero cells and the aggregation of LC3-Ⅱ into au-tophagosomes,suggesting that IBV could induce complete autophagy in Vero cells.The treatment with au-tophagy inhibitors,chloroquine and Wortmannin,inhibited IBV-induced autophagy and viral replication in Vero cells,meanwhile the treatment with autophagy activator rapamycin promoted viral replication.In summary,IBV could induce autophagy in Vero cells and promote viral replication.关键词
禽传染性支气管炎病毒/自噬/病毒复制Key words
avian infectious bronchitis virus/autophagy/virus replication分类
农业科技引用本文复制引用
杨雪晴,孙军峰,李慧昕,韩宗玺,刘胜旺..传染性支气管炎病毒诱导Vero细胞发生自噬促进病毒的复制[J].中国兽医科学,2024,54(8):1019-1026,8.基金项目
国家重点研发计划项目(2021YFD1801105) （2021YFD1801105）
黑龙江省自然科学基金团队项目(TD2021C001) （TD2021C001）
国家蛋鸡产业体系技术岗位科学家项目(CARS-40) （CARS-40）
