中国兽医科学2024，Vol.54Issue(8)：1027-1034,8.DOI:10.16656/j.issn.1673-4696.2024.0136
F81细胞外囊泡介导IFN-ω上调抗病毒细胞因子表达的研究
F81 extracellular vesicles mediate upregulation of antiviral cytokine expression by IFN-ω
摘要
Abstract
This study aims to investigate the role of extracellular vesicles(EVs)from feline kidney cells(F81)in mediating the regulation of antiviral cytokines through feline omega interferon(IFN-ω),providing a theoretical foundation for research on EV-mediated interferon antiviral mechanisms.IFN-ω-stimulated IFN-ω-EVs secreted by F81 cells were extracted and characterized for their marker proteins,morphology,and size using Western blotting,transmission electron microscopy,and nanoscale particle technology,respectively.F81 cells were incubated with IFN-ω-EVs containing IFN-ω,and fluorescence colocalization was analyzed using a laser confocal microscope to determine whether IFN-ω-EVs enter F81 cells.The expression levels of ISG15,ISG56,and IFN-ω cytokines were detected by RT-qPCR to investigate the antiviral effects of IFN-ω-EVs against VSV,in comparison with IFN-ω and a blank control.IFN-ω-EVs were primarily identified as cup-shaped vesicular structures carrying specific marker proteins CD63 and CD81,with particle sizes ranging between 50-100 nm and 250-600 nm.Fluorescence colocalization analysis revealed that IFN-ω-EVs could enter F81 cells.RT-qPCR results indicated that IFN-ω-EVs,like IFN-ω,significantly upregulated the expression levels of antiviral cytokines such as ISG15,ISG56,and IFN-ω.Importantly,the anti-VSV activity of IFN-ω-EVs post-VSV infection was significantly higher than that of IFN-ω alone(P＜0.05).IFN-ω-EVs extracted from F81 cells,can mediate the entry of IFN-ω by F81 cells,significantly upregulate the expression of antiviral cytokines such as ISG15,ISG56,and IFN-ω,and inhibit the inhibition of VSV virus proli-feration.关键词
细胞外囊泡/F81细胞/ω干扰素/水疱性口炎病毒Key words
extracellular vesicles/F81 cells/IFN-ω/vesicular stomatitis virus分类
农业科技引用本文复制引用
梁超,周雨霞,徐国伟,孙研,茹毅,李亚军,杨明星,张世栋,李建喜,王学智..F81细胞外囊泡介导IFN-ω上调抗病毒细胞因子表达的研究[J].中国兽医科学,2024,54(8):1027-1034,8.基金项目
甘肃省青年科技基金项目(21JR7RA035) （21JR7RA035）
兰州市人才创新产业项目(2022-RC-46) （2022-RC-46）
兰州市科技计划项目(2023-1-4) （2023-1-4）
中国农业科学院基础研究项目(Y2022XK19) （Y2022XK19）
