中国兽医科学2024，Vol.54Issue(8)：1056-1064,9.DOI:10.16656/j.issn.1673-4696.2024.0126
白斑综合征病毒DETECTR检测系统的建立
Establishment of DETECTR detection system for white spot syndrome virus
摘要
Abstract
To establish a rapid,sensitive and specific DNA endonuclease targeted CRISPR trans re-porter(DETECTR)detection system for white spot syndrome virus(WSSV)by combining recombinase-medi-ated amplification(RAA)with CRISPR-Cas12a.RAA primers and crRNA were designed for the conserved genes of WSSV,and the optimal combination and best reaction condition were screened experimentally to con-struct the WSSV DETECTR detection system.The sensitivity and specificity of the system were tested and compared with the real-time PCR and Nested PCR methods recommended by WOAH.The Results showed that the WSSV DETECTR system has a minimum detection limit of 10 copies/μL,which is the same as real-time PCR and Nested PCR(3.34×101 copies/μL),there was no cross-reactivity with other pathogens and no differ-ence in the test results from real-time PCR and Nested PCR for clinical samples.In conclusion,WSSV DE-TECTR detection system has the advantages of being rapid and simple,sensitive and specific,and pro-vides a new method for the rapid detection of white spot syndrome virus.关键词
重组酶介导等温扩增技术(RAA)/CRISPR-Cas12a/白斑综合征病毒/快速检测Key words
recombinase-mediated amplification/CRISPR-Cas12a/white sport syndrome virus/rapid detection分类
农业科技引用本文复制引用
徐博文,罗宝正,周傲白雪,廖秀云,蒋晓霞,邵建宏,沙才华,黄海超,赵福振,陈轩..白斑综合征病毒DETECTR检测系统的建立[J].中国兽医科学,2024,54(8):1056-1064,9.基金项目
广东省重点领域研发计划(2022B1111030001) （2022B1111030001）
