中国兽医科学2024，Vol.54Issue(8)：1096-1102,7.DOI:10.16656/j.issn.1673-4696.2024.0143
山羊环鸟苷酸-腺苷酸合成酶的原核表达及其酶活性分析
Prokaryotic expression and enzymatic activity of goat cyclic GMP-AMP synthase
摘要
Abstract
To investigate the biological functions of the goat cyclic GMP-AMP synthase(gcGAS)in innate immune response,the prokaryotic expression vector pET28a-SUMO-gcGAS was constructed and was transformed into Rosetta(DE3),and soluble expression was achieved through optimized condition.The recombinant gcGAS protein was purified and its enzymatic activity was tested in vitro.The results showed that the soluble recombinant gcGAS protein was expressed and it catalyzed the synthesis of 2'3'-cGAMP using ATP and GTP.In conclusion,the recombinant gcGAS protein had enzymatic activity,which laid a foundation of the molecular mechanism of the gcGAS in antiviral innate immunity and development of the cGAMP.关键词
环鸟苷酸-腺苷酸合成酶/环鸟苷酸-腺苷酸/原核表达/蛋白纯化/酶活性Key words
cyclic GMP-AMP synthase/cyclic GMP-AMP/prokaryotic expression/protein purifica-tion/enzymatic activity分类
农业科技引用本文复制引用
韦雨松,何小兵,张艳,田慧慧,翟军军,陈国华,房永祥,景志忠,屈雷,米晓云..山羊环鸟苷酸-腺苷酸合成酶的原核表达及其酶活性分析[J].中国兽医科学,2024,54(8):1096-1102,7.基金项目
新疆动物疫病研究重点实验室开放基金项目(2023KLB003) （2023KLB003）
国家自然科学基金项目(32060810,31960710) （32060810,31960710）
甘肃省自然科学基金项目(20JR10RA018,17JR5RA325) （20JR10RA018,17JR5RA325）
