中国兽医科学2024，Vol.54Issue(8)：1111-1118,8.DOI:10.16656/j.issn.1673-4696.2024.0134
甘宁地区蛋鸡源肺炎克雷伯菌的抗菌药物敏感性及耐药基因分析
Antibacterial susceptibility and resistance gene analysis of Klebsiella pneumoniae isolated from laying hens in Gansu and Ningxia
摘要
Abstract
Klebsiella pneumoniae is an opportunistic pathogen that can carry and disseminate antibacterial resistance genes via mobile genetic elements.This study aimed to isolate and identify K.pneumoniae from laying hens in Gansu and Ningxia.Antimicrobial susceptibility testing and detection of resistance phenotypes and genotypes were performed using the disc diffusion test and PCR,respectively.From 245 samples,we isolated 54 strains of K.pneumoniae,resulting in an isolation rate of 22.04％.All 54 strains exhibited the ability to form biofilms.The isolated strains demonstrated significant resistance to antibacterials such as spectinomycin,trimethoprim-sulfamethoxazole,tetracycline,enrofloxacin and ofloxacin,with 92.59％displaying a multidrug-resistant phenotype.High detection rates of resistance genes,including qnrB(90.74％),blaSHV(85.19％),floR(75.93％),sul1(72.22％),gyrA(70.37％),and tetA(64.81％),were observed,correlating with the resistance phenotype.Furthermore,we discovered a strain of multidrug-resistant K.pneumoniae carrying extended-spectrum β-lactamase(ESBL)genes blaSHV,blaTEM,blaCTX-M,and colistin resistance genes mcr-1 andmcr-8.Additionally,eight other resistance genes were identified,suggesting a compilation of diverse resistance genes mediated by mobile genetic elements.The findings of the current study underscore a concerning prevalence of K.pneumoniae in laying hens in both Gansu and Ningxia,signifying a critical situation regarding multidrug resistance and a comprehensive array of resistance genes.关键词
肺炎克雷伯菌/抗菌药物耐药性/耐药基因/蛋鸡Key words
Klebsiella pneumoniae/antibacterial resistance/resistance genes/laying hens分类
农业科技引用本文复制引用
严钰璋,茹梦珂,贺泂杰,王玲,王胜义,胡庭俊,崔东安..甘宁地区蛋鸡源肺炎克雷伯菌的抗菌药物敏感性及耐药基因分析[J].中国兽医科学,2024,54(8):1111-1118,8.基金项目
国家重点研发计划项目(2022YFD1801102 （）
2023YFD1800802) （）
兰州市科技计划项目(2022-2-61) （2022-2-61）
中央级公益性科研院所基本科研业务费专项(Y2022YJ14) （Y2022YJ14）
兽用天然药物与抗生素替代创新工程项目(25-LZIHPS-03) （25-LZIHPS-03）
