HPLC法同时测定槟榔四消丸中15种成分的含量OA北大核心CSTPCD
Simultaneous content determination of fifteen constituents in Binglang Sixiao Pills by HPLC
目的 建立HPLC法同时测定槟榔四消丸中没食子酸、儿茶素、表儿茶素、大黄酸-8-O-β-D-葡萄糖苷、绿原酸、大黄酚-8-O-β-D-葡萄糖苷、大黄素-8-O-β-D-葡萄糖苷、大黄素甲醚-8-O-β-D-葡萄糖苷、大黄酸、香附烯酮、木犀草素、α-香附酮、大黄素、大黄酚、大黄素甲醚的含量.方法 分析采用Kromasil C18 色谱柱(250 mm×4.6 mm,5 μm);流动相甲醇-0.1%甲酸,梯度洗脱;体积流量 1.0 mL/min;柱温 30℃;检测波长 260 nm.再进行聚类分析、主成分分析.结果 15 种成分在各自范围内线性关系良好(r＞0.999 0),平均加样回收率 96.00%～101.00%,RSD 0.50%～1.50%.15 批样品聚为 2 类,4 种主成分累积方差贡献率为 90.004%.结论 该方法简便准确,重复性好,可用于槟榔四消丸的质量控制.
AIM To establish an HPLC method for the simultaneous content determination of gallic acid,catechin,epicatechin,rhein 8-O-β-D-glucoside,chlorogenic acid,chrysophanol 8-O-β-D-glucoside,emodin-8-O-β-D-glucoside,emodin-3-methyl ether-8-O-β-D-glucoside,aloe emodin,rhein,cyperotundone,luteolin,α-cyperone,emodin,chrysophanol and emodin-3-methyl ether in Binglang Sixiao Pills.METHODS The analysis was performed on a 30℃thermostatic Kromasil C18 column(250 mm×4.6 mm,5 μm),with the mobile phase comprising of methanol-0.1%formic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 260 nm.Subsequently,cluster analysis and principal component analysis were made.RESULTS Fifteen constituents showed good linear relationships within their own ranges(r＞0.999 0),whose average recoveries were 96.00%-101.00%%with the RSDs of 0.50%-1.50%.Various batches of samples were clustered into two categories,and four principal components demonstrated the accumulative variance contribution rate of 90.004%.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Binglang Sixiao Pills.
王媛;杨元硕;李思治;赵佳萌
中国人民解放军联勤保障部队第九八三医院药剂科,天津 300142天津医科大学药学院,天津 300070
药学
槟榔四消丸化学成分含量测定HPLC聚类分析主成分分析
Binglang Sixiao Pillschemical constituentscontent determinationHPLCcluster analysisprincipal component analysis
《中成药》 2024 (008)
2493-2498 / 6
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