中成药2024，Vol.46Issue(8)：2506-2510,5.DOI:10.3969/j.issn.1001-1528.2024.08.003
HPLC法同时测定安神胶囊中7种成分的含量
Simultaneous content determination of seven constituents in Anshen Capsules by HPLC
摘要
Abstract
AIM To establish an HPLC method for the simultaneous content determination of spinosin,ferulic acid,rosmarinic acid,salvianolic acid B,schisandrin A,schizandrin B and schisandrol A in Anshen Capsules.METHODS The analysis was performed on a 30℃thermostatic Inertsustain C18 column(5 μm,4.6 mm×250 mm),with the mobile phase comprising of methanol-acetonitrile-0.1%phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 203,270 nm.Subsequently,principal component analysis and partial least squares discriminant analysis were made.RESULTS Seven constituents showed good linear relationships within their own ranges(r＞0.999 0),whose average recoveries were 96.12%-102.70%with the RSDs of 0.31%-1.83%.Ten batches of samples were divided into two categories according to manufacturers,and peaks 5(schisandrol A),4(salvianolic acid B)were quality difference markers.CONCLUSION This simple,sensitive,specific and reproducible method can be used for the quality control of Anshen Capsules.关键词
安神胶囊/化学成分/含量测定/HPLC/主成分分析/偏最小二乘判别分析Key words
Anshen Capsules/chemical constituents/content determination/HPLC/principal component analysis/partial least squares discriminant analysis分类
医药卫生引用本文复制引用
崔帅,崔俊凤,梁秀坤,王志勇,夏青..HPLC法同时测定安神胶囊中7种成分的含量[J].中成药,2024,46(8):2506-2510,5.基金项目
国家自然科学基金联合基金重点支持项目(U23A20514) （U23A20514）
