中成药2024，Vol.46Issue(8)：2517-2525,9.DOI:10.3969/j.issn.1001-1528.2024.08.005
UPLC-QTOF/MS法分析芪苈强心胶囊化学成分及入血成分
Analyses of chemical constituents and constituents absorbed into blood from Qili Qiangxin Capsules by UPLC-QTOF/MS
摘要
Abstract
AIM To establish a UPLC-QTOF/MS method for the analyses of chemical constituents and constituents absorbed into blood from Qili Qiangxin Capsules by UPLC-QTOF/MS.METHODS The rats with heart failure after myocardial infarction were given intragastric administration of 0.5%CMC-Na suspension of Qili Qiangxin Capsules(crude drug dosage was 0.9 g/mL)for 4 weeks,after which blood collection was made.The analysis was performed on a 40℃thermostatic Acquity UPLC BEH C18 Column(100 mm×2.1 mm,1.7 μm),with the mobile phase comprising of 0.1%formic acid-acetonitrile(positive ion manner)or water-acetonitrile(negative ion manner)flowing at 0.3 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning.RESULTS Total 151 constituents were identified,containing 38 flavonoids,36 terpenoids,23 alkaloids,11 saponins,11 phenylpropanol,10 phenolic acids,4 organic acids,4 cardiac glycosides and 14 others,along with 35 constituents absorbed into blood.CONCLUSION This accurate and stable method can provide data support for the rational clinical application of Qili Qiangxin Capsules,and lay foundation for the research on related effector substances of other Chinese traditional patent medicines.关键词
芪苈强心胶囊/化学成分/入血成分/UPLC-QTOF/MSKey words
Qili Qiangxin Capsules/chemical constituents/constituents absorbed into blood/UPLC-QTOF/MS分类
医药卫生引用本文复制引用
刘匡一,张凌云,熊彦婷,彭秘,陈晖..UPLC-QTOF/MS法分析芪苈强心胶囊化学成分及入血成分[J].中成药,2024,46(8):2517-2525,9.基金项目
江西省中医药管理局科技计划项目(2022B1067) （2022B1067）
