中成药2024，Vol.46Issue(8)：2571-2579,9.DOI:10.3969/j.issn.1001-1528.2024.08.012
妇科千金方及其拆方对盆腔炎性疾病后遗症大鼠的作用
Effects of Fuke Qianjin Formula and its modifications on the sequelae of pelvic inflammatory disease in rats
摘要
Abstract
AIM To study the effects and possible mechanism of Fuke Qianjin Formula and its modifications on sequelae of pelvic inflammatory disease(PID)in rats.METHODS The SD rats were randomly divided into the normal group,the sham operation group,the model group,Kangfuyan group(FYK,0.756 g/kg),Fuke Qianjin Formula group(FKQ,2.7 g/kg),Yijun Formula group(YJ,2.7 g/kg),Kangyan Formula group(KY,2.7 g/kg)and Buyi Formula group(BY,2.7 g/kg).After 28 days of corresponding administration by gavage,the rats were put to death to procure their uterus,spleen,thymus and liver for the calculation of the organs indices levels;the observation of the general morphology of uterus and oviduct and histopathological examination of uterus and oviduct;the measurement of.the serum levels of IL-1β,IL-18 and IL-6 by ELISA;the detection of the uterine expressions of TGF-β1,caspase-1,NLRP3,IL-18 and CTGF by Western blot;and the detection of the expressions of CD4+and CD8+in spleen lymphocytes by flow cytometry.RESULTS Compared with the model group,FKQ and YJ group,KY group and BY group displayed decreased indices levels of the uterus,spleen and thymus(P＜0.01);FKQ group and KY group displayed reduced uterine swelling,increased endometrial glands,and reduced inflammatory exudation(P＜0.01),and lower serum levels of IL-1β,IL-18 and IL-6(P＜0.01);FKQ group,KYgroup and BY group displayed decreased protein expressions of TGF-β1 and CTGF(P＜0.05,P＜0.01);FKQ group and KY group displayed decreased protein expressions of NLRP3,caspase-1 and IL-18(P＜0.05,P＜0.01);and FKQ group and BY group displayed increased CD4+/CD8+ratio(P＜0.01).CONCLUSION Being protective to sequelae of PID rat models,Fuke Qianjin Formula and its modifications may contribute their anti-inflammatory efficacy by inhibiting the activation of NLRP3 body to hinder the activation of caspase-1,reduce the secretion and release of pro-inflammatory factor IL-18,and thus inhibit pyroptosis.关键词
妇科千金方及其拆方/盆腔炎性疾病后遗症/抗炎/细胞焦亡Key words
Fuke Qianjin Formula and its modifications/sequelae of pelvic inflammatory disease/anti-inflammatory/pyroptosis分类
医药卫生引用本文复制引用
徐佳,谭雅莉,肖志葵,匡继林,林丽美,雷磊..妇科千金方及其拆方对盆腔炎性疾病后遗症大鼠的作用[J].中成药,2024,46(8):2571-2579,9.基金项目
国家中药标准化项目(ZYBZH-C-HUN-21) （ZYBZH-C-HUN-21）
湖南省中医药管理局重点项目(2020011) （2020011）
湖南省自然科学基金(2022JJ80085) （2022JJ80085）
湖南中医药大学科研基金项目(2022XYLH027) （2022XYLH027）
湖南中医药大学校级研究生创新课题(2021CX52) （2021CX52）
