中成药2024，Vol.46Issue(8)：2617-2623,7.DOI:10.3969/j.issn.1001-1528.2024.08.021
山橿根大极性部位化学成分及其抗肿瘤活性研究
Chemical constituents from the large polar fraction of the roots of Lindera reflexa and their antitumor activities
摘要
Abstract
AIM To study the chemical constituents from the large polar fraction of the roots of Lindera reflexa Hemsl.and their antitumor activities.METHODS The large polar fraction from the roots of L.reflexa was isolated and purified by silica gel column,Sephadex LH-20 gel column,semi-preparative HPLC and ODS medium pressure column,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The antitumor activities were determined by MTT method.RESULTS Thirteen compounds were isolated and identified as 2,6-dimethoxy-4-hydroxyphenyl-1-O-β-D-glucopyranoside(1),3-hydroxy-4,5-dimethoxyphenol-β-D-glucopyranoside(2),syringin(3),1-O-3,4-dimethoxy-5-hydroxyphenyl-(6-O-3,5-dimethoxygalloyl)-β-D-glucopyranoside(4),p-cymen-7-yl β-D-glucopyranoside(5),pisumionoside(6),staphylionoside D(7),dendranthemoside B(8),lynoiside(9),nudiposide(10),icariside B1(11),(2S)-pinocembrin-7-O-(6-O-α-L-rhamnopyranosyl-β-D-glucopyranoside)(12),(+)-N-(methoxycarbonyl)-N-norboldine(13).Compounds 3 and 13 showed obvious cytotoxicity against human lung cancer cells(A549)and human gastric cancer cells(MGC80-3).CONCLUSION Compounds 1-13 are isolated from the roots of L.reflexa for the first time.Compounds 3 and 13 have good anti-tumor activities.关键词
山橿/根/化学成分/分离鉴定/抗肿瘤活性Key words
Lindera reflexa Hemsl/roots/chemical constituents/isolation and identification/antitumor activities分类
医药卫生引用本文复制引用
田志浩,孙孝亚,张珊珊,郜志宁,李焱,陈若曦,陈随清..山橿根大极性部位化学成分及其抗肿瘤活性研究[J].中成药,2024,46(8):2617-2623,7.基金项目
国家自然科学基金项目(81773859) （81773859）
河南省科技攻关项目(212102310353) （212102310353）
河南省中药材产业技术体系(豫财科[2023]7 号) （豫财科[2023]7 号）
