中成药2024，Vol.46Issue(8)：2623-2630,8.DOI:10.3969/j.issn.1001-1528.2024.08.022
来江藤苯丙素类成分及其抗氧化活性研究
Phenylpropanoids from Brandisia hancei and their antioxidant activities
摘要
Abstract
AIM To study the phenylpropanoids from Brandisia hancei Hook.f.and their antioxidant activities.METHODS The extract from B.hancei was isolated and purified by Rp-C18,MCI,semi-preparative HPLC,silica gel and Sephadex LH-20,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The cytotoxicities was determined by MTT method,and the antioxidant activities were determined by DPPH and ABTS+free radical scavenging methods.RESULTS Fifteen phenylpropanoids were isolated and identified as(+)-pinonesinol(1),(-)-medioresinol(2),(-)-syringaresinol(3),buddlenol D(4),(7R,7'R,7″S,8S,8'S,8″S)-4',5″-dihydroxy-3,5,3',4″-tetramethoxy-7,9':7',9-diepoxy-4,8″-oxy-8,8'-sesquineo-lignan-7″,9″-diol(5),(-)-(7R,7'R,7″R,8S,8'S,8″S)-4',4″-dihydroxy-3,3',3″,5-tetramethoxy-7,9':7',9-diepoxy-4,8″-oxy-8,8'-sesquineolignan-7″,9″-diol(6),hedyotol A(7),dracunculifoside R(8),acteoside(9),isoacteoside(10),arenarioside(11),isomartynoside(12),curcasinlignan B(13),erythro-2,3-bis(4-hydroxy-3-methoxyphenyl)-3-ethoxypropan-l-ol(14),citrusin C(15).Compounds 1-4 and 9-10 had no obvious cytotoxicity to HepG2 hepatoma cells.Compounds 1,3,9,10 and 12 had strong scavenging activities against DPPH radicals.Compounds 1-3,9-10,12 and 14 showed strong scavenging activities against ABTS+radical.CONCLUSION Compounds 1-8 and 12-15 are isolated from genus Brandisia for the first time.The phenylpropanoids from B.hancei show strong antioxidant activities.关键词
来江藤/苯丙素/分离鉴定/细胞毒性/抗氧化活性Key words
Brandisia hancei Hook.f./phenylpropanoids/isolation and identification/cytotoxicities/antioxidant activities分类
医药卫生引用本文复制引用
李昌芬,廖彬彬,刘宗绪,王洪云,张新渐,左爱学..来江藤苯丙素类成分及其抗氧化活性研究[J].中成药,2024,46(8):2623-2630,8.基金项目
云南省科学技术厅-云南中医学院应用基础研究联合专项基金(202101AZ070001-218) （202101AZ070001-218）
云南省南药可持续利用研究重点实验室2021 年开放课题(202105AG070012ZD2203) （202105AG070012ZD2203）
云南省教育厅治疗痹症中医中药工程研究中心(培育) （培育）
保山市科学技术局科技计划项目"保山市中青年学术技术带头人"(202003) （202003）
兴滇英才支持计划项目(20220273) （20220273）
