中成药2024，Vol.46Issue(8)：2630-2637,8.DOI:10.3969/j.issn.1001-1528.2024.08.023
白鹤灵芝化学成分及其体外抗肿瘤与降脂活性研究
Chemical constituents from Rhinacanthus nasutus and their in vitro antitumor and lipid-lowering activities
摘要
Abstract
AIM To study the Chemical constituents from Rhinacanthus nasutus(L.)Kurz and their in vitro antitumor and lipid-lowering activities.METHODS The ethyl acetate fraction from R.nasutus was isolated and purified by normal phase,reverse phase silica gel column and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The in vitro anti-tumor activity was determined by MTT assay,and the in vitro lipid-lowering activity was evaluated by oleic acid-induced HepG2 high-fat cell model.RESULTS Twenty compounds were isolated and identified as rhinacanthin A(1),rhinacanthin B(2),rhinacanthin C(3),rhinacanthin D(4),rhinacanthin E(5),rhinacanthin M(6),rhinacanthin N(7),rhinacanthin Q(8),rhinacanthin T(9),rhinacanthone(10),β-sitosterol(11),gallic acid(12),vanillic acid(13),syringic acid(14),lapachol(15),umbelliferone(16),sambucunlin A(17),17α,21-dihydroxy-1,4-pregnadiene-3,11,20-trione,21-acetate(18),1,8-dihydroxyanthraquinone(19),2,6-dimethoxy-1,4-benzoquinone(20).Compounds 3 and 7 inhibited the proliferation of HepG2,Hela,A549 and H22 tumor cells with IC50 values of(0.66±0.17)-(3.22±0.49)μmol/L in a time-and concentration-dependent manner.Compounds 3,7 and 9 had a lipid clearance rate of more than 50%in oleic acid-induced HepG2 high-fat cells.CONCLUSION Compounds 16-20 are isolated from this plant for the first time.Compounds 3 and 7 have good in vitro anti-tumor activities,and 3,7,9 have good in vitro lipid-lowering activities.关键词
白鹤灵芝/化学成分/分离鉴定/抗肿瘤/降脂Key words
Rhinacanthus nasutus(L.)Kurz/chemical constituents/isolation and identification/anti-tumor/lipid-lowering分类
医药卫生引用本文复制引用
龚志强,肖阳,韩沙,刘颖,齐晋,霍宇,黄艳红,杨立芳..白鹤灵芝化学成分及其体外抗肿瘤与降脂活性研究[J].中成药,2024,46(8):2630-2637,8.基金项目
广西自然科学基金项目(2018GXNSFBA281033) （2018GXNSFBA281033）
广西壮瑶药重点实验室(桂科基字[2014]32 号,22-035-25) （桂科基字[2014]32 号,22-035-25）
壮瑶药协同创新中心(桂教科研[2013]20 号) （桂教科研[2013]20 号）
广西高校中青年教师基础能力提升项目(2021KY1684,2022KY1675) （2021KY1684,2022KY1675）
校级科研项目(2022MS004) （2022MS004）
