中成药2024，Vol.46Issue(8)：2638-2644,7.DOI:10.3969/j.issn.1001-1528.2024.08.024
金银花化学成分及其抗氧化活性研究
Chemical constituents from Lonicera japonica and their antioxidant activities
摘要
Abstract
AIM To study the chemical constituents from Lonicera japonica Thunb.and their antioxidant activities.METHODS The extract from L.japonica was isolated and purified by D101 macroporous resin,silica gel column and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The antioxidant activity was determined by DPPH free radical scavenging method.RESULTS Fifteen compounds were isolated and identified as dearabinosyl pneumonanthoside(1),5α-carboxystrictosidine(2),apigenin 7-O-glucoside(3),ethyl caffeate(4),isorhamnetin-3-O-β-D-glucopyranoside(5),luteolin 7-O-glucoside(6),quercetin 3-O-β-glucoside(7),luteolin 7-O-rutinoside(8),luteolin-7-O-neohesperidoside(9),hydnocarpin(10),methyl chlorogenate(11),(Z)-aldosecolohanin(12),kaempferol 3-O-β-D-glucoside(13),chlorogenic acid butyl ester(14),(-)-syringaresinol(15).The IC50 values of DPPH free radical scavenging of compounds 3,5-10,13-14 were 6.70-36.25 μmol/L.CONCLUSION Compounds 1-2,8,9-11 and 15 are isolated from genus Lonicera for the first time.Compounds 3,5-10,13-14 show good antioxidant activities.关键词
金银花/化学成分/分离鉴定/抗氧化活性Key words
Lonicera japonica Thunb./chemical constituents/isolation and identification/antioxidant activities分类
医药卫生引用本文复制引用
陆远,张研,王莹,董佳璇,杜宝玉,张永清,杨然..金银花化学成分及其抗氧化活性研究[J].中成药,2024,46(8):2638-2644,7.基金项目
中央引导地方科技发展资金项目(XZ202301YD0023C) （XZ202301YD0023C）
中央本级重大增减支项目(2060302) （2060302）
山东省重点研发计划(2021CXGC010510,2022CXGC010511) （2021CXGC010510,2022CXGC010511）
山东中医药大学药学院青年教师科研强基计划(2021-0012,2021-0015) （2021-0012,2021-0015）
