中成药2024，Vol.46Issue(8)：2644-2649,6.DOI:10.3969/j.issn.1001-1528.2024.08.025
黄芩内生真菌及其次级代谢产物酶抑制活性研究
Endophytic fungi from Scutellaria baicalensis and the enzyme inhibitory activities of their secondary metabolites
摘要
Abstract
AIM To study endophytic fungi from Scutellaria baicalensis Georgi.and the enzyme inhibitory activities of their secondary metabolites.METHODS Six different media were used to isolate and purify endophytic fungi from S.baicalensis by tissue homogenate method.The activities of secondary metabolites were evaluated by targeting different enzymes.The highly active strains were identified by molecular biology combined with morphology,and the highly active chemical components were tracked and separated by modern chromatographic separation technology.RESULTS Sixty-four endophytic fungal strains were isolated from S.baicalensis,and one hundred and twenty-eight secondary metabolites were obtained by fermentation.The samples with certain inhibitory activities against adenosine deaminase(ADA),β-lactamase and tyrosinase(TYR)accounted for 14.06%,3.91%and 18.75%,respectively.Strain HTS-23-2 showed high TYR inhibitory activity,and 99%homology with Aspergillus flavus by molecular identification.One compound was isolated from the fermentation samples and identified as kojic acid.CONCLUSION S.baicalensis harbors a rich diversity of endophytic fungi,which serve as a valuable resource for active substances.关键词
黄芩/内生真菌/代谢产物/酶抑制活性/跟踪分离Key words
Scutellaria baicalensis Georgi/endophytic fungi/metabolites/enzyme inhibitory activities/tracking separation分类
医药卫生引用本文复制引用
李德民,马小弟,王康旭,李媚媛,罗满平,孟瑛瑛,杨爱梅,王蓓,张新国..黄芩内生真菌及其次级代谢产物酶抑制活性研究[J].中成药,2024,46(8):2644-2649,6.基金项目
国家自然科学基金(32160021) （32160021）
甘肃省重点研发计划项目(21YF5FA084,22YF7NA176) （21YF5FA084,22YF7NA176）
甘肃省科技专员专项(22CX8GA114) （22CX8GA114）
温州市科技局(2022Y0883) （2022Y0883）
甘肃省青年基金计划(22JR5RA299) （22JR5RA299）
