作物学报2024，Vol.50Issue(9)：2157-2166,10.DOI:10.3724/SP.J.1006.2024.44018
花生干旱诱导型启动子AhMYB44-11-Pro的克隆与功能分析
Cloning and functional analysis of drought-inducible promoter AhMYB44-11-Pro in peanut(Arachis hypogaea L.)
摘要
Abstract
Drought is a significantly environmental factor that poses a serious threat to peanut yield and quality in China.Our study aims to elucidate the regulatory mechanism of the drought stress response gene AhMYB44-11 and uncover the function of its promoter.We isolated the promoter sequence AhMYB44-11-Pro from the peanut genome and constructed recombinant expression vectors comprising the full-length promoter and several 5'-terminal deletion promoters,each fused with the GUS reporter gene.These vectors were then introduced into plant tissue via Agrobacterium rhizogenes-mediated transformation to assess promoter activity and expression patterns.The results revealed that AhMYB44-11-Pro contains a higher number of drought-responsive cis-regulatory elements,including MBS and Myb-binding sites,compared to the promoter of its homologous gene AhMYB44-01.Furthermore,dehydration treatment significantly enhanced GUS staining and activity in AhMYB44-11-Pro transgenic Arabidopsis,indicating that the promoter's activity is upregulated under drought stress.These findings confirm that AhMYB44-11-Pro functions as a drought-inducible promoter.Additionally,AhMYB44-11-Pro contains a seed endosperm-specific expression element and gib-berellin response elements,demonstrating an increased expression trend during pod development,as evidenced by GUS histo-chemical staining.This suggests that AhMYB44-11 may play a crucial role in seed development,particularly in dry matter accu-mulation.This research lays the groundwork for a comprehensive analysis of the biological functions of AhMYB44 and provides a valuable reference for genetically enhancing crop drought resistance.关键词
花生/AhMYB44/启动子/干旱胁迫/GUS活性Key words
peanut/AhMYB44/promoter/drought stress/GUS activity引用本文复制引用
刘永惠,沈一,沈悦,梁满,沙琴,张旭尧,陈志德..花生干旱诱导型启动子AhMYB44-11-Pro的克隆与功能分析[J].作物学报,2024,50(9):2157-2166,10.基金项目
本研究由财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-13)和江苏省种业振兴揭榜挂帅项目(JBGS[2021]062)资助.This study was supported by the China Agriculture Research System of MOF and MARA(CARS-13)and the Open Competition Project for Seed Industry of Jiangsu Province(JBGS[2021]062). （CARS-13）
