花生干旱诱导型启动子AhMYB44-11-Pro的克隆与功能分析OA北大核心CSTPCD

Drought is a significantly environmental factor that poses a serious threat to peanut yield and quality in China.Our study aims to elucidate the regulatory mechanism of the drought stress response gene AhMYB44-11 and uncover the function of its promoter.We isolated the promoter sequence AhMYB44-11-Pro from the peanut genome and constructed recombinant expression vectors comprising the full-length promoter and several 5'-terminal deletion promoters,each fused with the GUS reporter gene.These vectors were then introduced into plant tissue via Agrobacterium rhizogenes-mediated transformation to assess promoter activity and expression patterns.The results revealed that AhMYB44-11-Pro contains a higher number of drought-responsive cis-regulatory elements,including MBS and Myb-binding sites,compared to the promoter of its homologous gene AhMYB44-01.Furthermore,dehydration treatment significantly enhanced GUS staining and activity in AhMYB44-11-Pro transgenic Arabidopsis,indicating that the promoter's activity is upregulated under drought stress.These findings confirm that AhMYB44-11-Pro functions as a drought-inducible promoter.Additionally,AhMYB44-11-Pro contains a seed endosperm-specific expression element and gib-berellin response elements,demonstrating an increased expression trend during pod development,as evidenced by GUS histo-chemical staining.This suggests that AhMYB44-11 may play a crucial role in seed development,particularly in dry matter accu-mulation.This research lays the groundwork for a comprehensive analysis of the biological functions of AhMYB44 and provides a valuable reference for genetically enhancing crop drought resistance.