作物学报2024，Vol.50Issue(9)：2167-2178,12.DOI:10.3724/SP.J.1006.2024.31081
小麦农家种成株期条锈病抗性QTL定位及其育种效应解析
Mapping of QTL for adult plant stripe rust resistance genes in a Sichuan wheat landrace and the evaluation of their breeding effects
摘要
Abstract
Stripe rust,caused by Puccinia striiformis f.sp.tritici(Pst),is a worldwide wheat disease that causes large losses in production.The lack of in-depth understanding of genetic and breeding utilization effects,coupled with the linkage of undesirable traits,are the key factors that limit the wide application of most of the discovered wheat stripe rust resistance genes in breeding and production.Our previous studies have shown that the wheat landraces,Hongmangmaizi(HM),exhibited stable adult plant stripe rust resistance to prevalent physiological races and pathogenic groups of stripe rust in China.In order to reveal the genetic basis of HM adult plant resistance to stripe rust,F1,F2,and F2:3 segregating populations derived from the cross between Avocet S and HM were inoculated with mixed Pst and evaluated for stripe rust reaction at adult stage in the field.Through bulked segrega-tion analyses(BSA)using 55K single-nucleotide polymorphism array and exon sequencing technology,we identified two quanti-tative trait loci(QTL)on chromosomes 7AL and 7DS,designated as QYr.HM-7AL and QYr.HM-7DS,explaining 11.64%-15.25%and 24.33%-40.58%of the phenotypic variance,respectively.Through integrated linkage,genetic and physical map analyses indicated that the major QTL QYr.HM-7DS was corresponding to the Yr18 gene,whereas QYr.HM-7AL,which was the second most stable QTL,should be a potential novel QTL for adult-stage stripe rust resistance.Furthermore,the kompetitive allele-specific PCR(KASP)markers,tightly linked with QYr.HM-7AL,were developed and validated.Based on construction of BC1F2 genetic improvement population derived from a cross between Mianmai 1618×HM,the main QTL genetic effect of stripe rust resistance and its synergistic effect on yield related traits were analyzed.The results showed that the two QTL(Yr18 and QYr.HM-7AL)could significantly reduce the damage of stripe rust and had a positive effect on spike length and tillering number under the genetic background of Mianmai 1618.The above results indicated that the QTLs of adult-stage stripe rust resistance from HM could be used in wheat yield breeding programs.关键词
小麦农家种/成株期条锈病抗性基因/红芒麦子/遗传效应/育种效应Key words
wheat landrace/adult plant stripe rust resistance genes/Hongmangmaizi/genetic effect/breeding effect引用本文复制引用
黄林玉,张潇月,李豪,邓梅,康厚扬,魏育明,王际睿,蒋云峰,陈国跃..小麦农家种成株期条锈病抗性QTL定位及其育种效应解析[J].作物学报,2024,50(9):2167-2178,12.基金项目
本研究由国家农业重大科技专项(NK20220607),国家自然科学基金项目(32272059),四川省重大科技专项(2022ZDZX0014)和四川省重点研发项目(2021YFYZ0002)资助.The study was supported by the Major Program of National Agricultural Science and Technology of China(NK20220607),the National Natural Science Foundation of China(32272059),the Major Science and Technology Project of Sichuan Province(2022ZDZX0014),and the Key Research and Development Project of Sichuan Province(2021YFYZ0002). （NK20220607）
