大豆种质资源苗期耐盐性鉴定评价与筛选
Identification,evaluation and screening of salt-tolerant of soybean germplasm resources at seedling stage
摘要
Abstract
Soil salinization has a significant impact on soybean quality and yield.Therefore,it is crucial to screen salt-tolerant soybean resources for the comprehensive utilization of salinized land.To establish a method for identifying and evaluating salt tolerance in soybean at the seedling stage,we conducted experiments using fresh water and 10 equal-differential gradients of NaCl solutions ranging from 0.9%to 1.8%.These solutions were applied to vermiculite culture medium when the soybean plants reached the stage of two emerged leaves.Results indicated that the 1.2%salinity treatment for 16 days demonstrated the highest interquartile range of salt-tolerant grading among various soybean germplasm resources,thus considered the optimal condition for evaluating salt tolerance in soybean seedlings.A total of 504 soybean germplasm resources from diverse geographic regions at home and abroad were subjected to evaluation using the soybean salt-tolerant evaluation system.Among them,46,146,157,79,and 76 soybean germplasm resources received salt-tolerant gradings of 1,2,3,4,and 5,respectively.The soybean resources with salt-tolerant grading 1 were further analyzed using the GmSALT3 gene molecular marker.The amplification results of 40 soybean resources(80.96%)were consistent with the GmSALT3 gene molecular marker.To analyze the change in salt stress concentration in the culture medium during the identification of soybean seedlings,a regression equation was established between soil salt con-tent(Y,%)and electrical conductivity(X,mS cm-1):Y=0.278X-0.0618,with a prediction accuracy above 95%.The variation trend of salt content in the culture medium was measured from the beginning of salt treatment until the end of the investigation,and it was found to be maintained at approximately 13 mS cm-1.This study not only provides a technical system for large-scale identification of salt-tolerant soybean seedlings but also serves as a foundation for breeding new soybean salt-tolerant germplasm resources.关键词
大豆种质资源/苗期耐盐体系/苗期耐盐资源/土壤含盐量测定Key words
soybean germplasm resources/salt-tolerant system at seedling stage/salt-tolerant germplasm resources at seedling stage/soil salinity determination引用本文复制引用
孙现军,胡正,姜雪敏,王世佳,陈向前,张惠媛,张辉,姜奇彦..大豆种质资源苗期耐盐性鉴定评价与筛选[J].作物学报,2024,50(9):2179-2186,8.基金项目
本研究由国家重点研发计划项目(2021YFD1201603),山东省重点研发计划项目(ZDYF2023LZGC001),海南省重点研发计划项目(ZDYF2022XDNY135),中国农业科学院科技创新工程项目(ASTIP No.CAAS ZDRW202201,01-ICS-02),国家自然科学基金项目(31601329)和江苏省现代作物生产协同创新中心和现代作物生产省部共建协同创新中心项目(CIC-MCP)资助.This study was supported by the National Key Research and Development Program of China(2021YFD1201603),the Key Research and Development Program of Shandong Province(ZDYF2023LZGC001),the Key Research and Development Program of Hainan Province(ZDYF2022XDNY135),the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(ASTIP No.CAAS ZDRW202201,01-ICS-02),the National Natural Science Foundation of China(31601329),and the Collaborative Innova-tion Center for Modern Crop Production co-sponsored by Province and Ministry(CIC-MCP). （2021YFD1201603）
