作物学报2024，Vol.50Issue(9)：2207-2218,12.DOI:10.3724/SP.J.1006.2024.34216
谷子育成品种萌芽期耐冷性综合评价
Comprehensively evaluation on cold tolerance of foxtail millet varieties at germination stage
摘要
Abstract
Heilongjiang province,located in high latitudes,frequently experiences cold damage during the germination period of millet after sowing.This leads to slow and uneven emergence,as well as insufficient seedling growth,which is a key limiting factor for millet yield and quality in this region.Identifying the cold tolerance of millet and screening appropriate evaluation indi-ces for cold tolerance can provide a theoretical foundation for breeding cold-tolerant millet varieties.It holds great significance for ensuring the safe production of millet.In this study,52 foxtail millet cultivars were selected as research subjects.Twelve cold stress treatments were applied during the germination stage,and the suitability of using germination rate as the index for evaluat-ing cold tolerance at this stage was explored.Following low-temperature stress,the cold tolerance coefficients of seven indices,including germination potential,germination rate,germination index,bud length,root length,bud fresh weight,and root fresh weight,were measured.Comprehensive evaluation of the tested varieties'cold tolerance was conducted using membership func-tion analysis,principal component analysis,correlation analysis,and cluster analysis.Stepwise regression analysis was employed to establish a regression equation for predicting cold tolerance.The results revealed that the germination rate of millet varieties exhibited the largest variation range after seven days of low-temperature treatment at 8℃.This treatment effectively distinguished the differences in cold tolerance among varieties and proved to be the most suitable condition for evaluating cold tolerance during the germination stage.Following low-temperature stress,all seven evaluated indices showed lower values compared to the control treatment at 28℃,although the degree of reduction varied among the indices.Through principal component analysis,the seven individual indices were transformed into three independent comprehensive indices.It was found that bud length,bud fresh weight,and root fresh weight best represented the response of millet during the germination period to low-temperature stress.These indi-ces can be used for evaluating cold tolerance during the germination stage.Cluster analysis categorized the 52 millet varieties into three groups,identifying 17 highly cold-tolerant varieties such as Nenxuan 14,Chaogu 14,and Mengheigu 8,as well as 15 cold-sensitive varieties such as Lugu 7 and Jingu 20.These varieties serve as valuable germplasm resources for breeding cold-tolerant millet and enhancing millet production.关键词
谷子/萌芽期/冷胁迫/综合评价/主成分分析Key words
foxtail millet/germination stage/cold stress/comprehensive evaluation/principal components analysis引用本文复制引用
闫锋,刘凯,董扬,李清泉,赵富阳,侯晓敏,刘洋,李青超,赵蕾,范国权..谷子育成品种萌芽期耐冷性综合评价[J].作物学报,2024,50(9):2207-2218,12.基金项目
本研究由财政部和农业农村部国家农业现代产业技术体系建设专项(CARS-06-14.5-B21)资助.This study was supported by the China Agricultural Research System of MOF and MARA(CARS-06-14.5-B21). （CARS-06-14.5-B21）
