珍珠粟WD40基因家族鉴定及表达特征分析OA北大核心CSTPCD
Identification and expression analysis of the WD40 gene family in pearl millet
珍珠粟是世界范围重要的谷物,拥有极强的光合能力和高生产潜力,较其他作物相比,珍珠粟具有对贫瘠土壤的耐受性,能够适应多种非生物胁迫和多样化的环境条件.PgWD40 基因家族在植物抵御生物和非生物胁迫以及调控植物生长发育中扮演着重要角色.本研究利用生物信息学方法全面鉴定和分析了珍珠粟中的PgWD40 基因家族及其表达模式.研究结果显示,共鉴定出 209 个 PgWD40 基因家族成员,通过对珍珠粟和水稻的系统进化分析将其归为 5 个亚家族,在同一亚族内的成员中,它们的保守序列和基因结构表现出一定的相似性.此外,通过对启动子顺式作用元件的分析显示,176 个PgWD40 基因与植物的生长和发育相关,208 个PgWD40 基因成员含有不同激素胁迫响应的顺式作用元件,进一步通过转录组数据分析和 qRT-PCR 分析显示,PMA3G03393.1、PMA4G00558.1、PMA5G02217.1 等基因受到盐、热和干旱胁迫的诱导,表明这些基因可能通过依赖不同激素的信号通路来调控和响应非生物胁迫,可作为进一步研究PgWD40 基因家族耐受性功能的候选基因.并且PgWD40 基因家族成员在珍珠粟抽穗期的不同时期存在表达差异.通过基因表达热图以及GO和KEGG等分析,发现许多PgWD40 基因家族成员参与了植物生长发育和籽粒形成的各个阶段.研究结果为全面解析PgWD40 基因结构与生物学功能、耐逆性分子机制以及分子育种提供了理论基础,为今后培育高效抗逆作物新品种提供基因资源.
Pearl millet is a globally significant cereal,known for its excellent photosynthetic capabilities and high production potential.It has evolved tolerance to infertile soils and the ability to adapt to various abiotic stressors and diverse environmental conditions,setting it apart from other crops.The PgWD40 gene family plays a crucial role in plant defense against biotic and abiotic stresses,as well as in the regulation of plant growth and development.In this study,we conducted a comprehensive identi-fication and analysis of the PgWD40 gene family and its expression pattern in pearl millet.A total of 209 members of the PgWD40 gene family were identified and categorized into five subfamilies through phylogenetic analysis of pearl millet and rice.Members within the same subfamily exhibited some similarity in their conserved sequences and gene structures.Furthermore,analysis of promoter cis-acting elements revealed that 176 PgWD40 genes were associated with plant growth and development,while 208 PgWD40 gene members contained cis-acting elements related to different hormone stress responses.Transcriptomic data analysis and qRT-PCR analysis indicated that PMA3G03393.1,PMA4G00558.1,and PMA5G02217.1 were induced by salt,heat,and drought stresses,suggesting their involvement in regulating and responding to abiotic stresses through hor-mone-dependent signaling pathways.These genes hold potential for further studies on the tolerance function of the PgWD40 gene family.Additionally,the PgWD40 gene family exhibited differential expression during different stages of pearl millet tasseling.Through gene expression heat maps,as well as GO and KEGG analysis,it was found that many members of the PgWD40 gene family are involved in various stages of plant growth,development,and seed formation.The results of this study provide a theo-retical basis for comprehensive analysis of the structure and biological function of the PgWD40 gene,as well as understanding the molecular mechanisms underlying stress tolerance and molecular breeding.Furthermore,it offers valuable genetic resources for the cultivation of new high-efficiency stress-resistant crop varieties.
杨煜琛;靳雅荣;骆金婵;祝鑫;李葳航;贾纪原;王小珊;黄德均;黄琳凯
四川农业大学草业科技学院,四川成都 611130重庆市畜牧科学院,重庆 402460
珍珠粟WD40基因家族生物信息学分析产量逆境胁迫
Pearl milletWD40 gene familybioinformatics analysisyieldadversity stress
《作物学报》 2024 (009)
2219-2236 / 18
本研究由重庆市财政专项资金项目(23515C),重庆市自然科学基金项目(CSTB2022NSCQ-MSX0274),四川省饲草创新团队项目(SCCXTD-2020-16)和四川省育种攻关项目(2021YFYZ0013)资助.This study was supported by the Chongqing Municipal Special Funds Project(23515C),the Chongqing Natural Science Foundation Project(CSTB2022NSCQ-MSX0274),the Sichuan Province Forage Innovation Team Project(SCCXTD-2020-16),and the Sichuan Province Breeding Tackling Project(2021YFYZ0013).
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