作物学报2024，Vol.50Issue(9)：2219-2236,18.DOI:10.3724/SP.J.1006.2024.34214
珍珠粟WD40基因家族鉴定及表达特征分析
Identification and expression analysis of the WD40 gene family in pearl millet
摘要
Abstract
Pearl millet is a globally significant cereal,known for its excellent photosynthetic capabilities and high production potential.It has evolved tolerance to infertile soils and the ability to adapt to various abiotic stressors and diverse environmental conditions,setting it apart from other crops.The PgWD40 gene family plays a crucial role in plant defense against biotic and abiotic stresses,as well as in the regulation of plant growth and development.In this study,we conducted a comprehensive identi-fication and analysis of the PgWD40 gene family and its expression pattern in pearl millet.A total of 209 members of the PgWD40 gene family were identified and categorized into five subfamilies through phylogenetic analysis of pearl millet and rice.Members within the same subfamily exhibited some similarity in their conserved sequences and gene structures.Furthermore,analysis of promoter cis-acting elements revealed that 176 PgWD40 genes were associated with plant growth and development,while 208 PgWD40 gene members contained cis-acting elements related to different hormone stress responses.Transcriptomic data analysis and qRT-PCR analysis indicated that PMA3G03393.1,PMA4G00558.1,and PMA5G02217.1 were induced by salt,heat,and drought stresses,suggesting their involvement in regulating and responding to abiotic stresses through hor-mone-dependent signaling pathways.These genes hold potential for further studies on the tolerance function of the PgWD40 gene family.Additionally,the PgWD40 gene family exhibited differential expression during different stages of pearl millet tasseling.Through gene expression heat maps,as well as GO and KEGG analysis,it was found that many members of the PgWD40 gene family are involved in various stages of plant growth,development,and seed formation.The results of this study provide a theo-retical basis for comprehensive analysis of the structure and biological function of the PgWD40 gene,as well as understanding the molecular mechanisms underlying stress tolerance and molecular breeding.Furthermore,it offers valuable genetic resources for the cultivation of new high-efficiency stress-resistant crop varieties.关键词
珍珠粟/WD40基因家族/生物信息学分析/产量/逆境胁迫Key words
Pearl millet/WD40 gene family/bioinformatics analysis/yield/adversity stress引用本文复制引用
杨煜琛,靳雅荣,骆金婵,祝鑫,李葳航,贾纪原,王小珊,黄德均,黄琳凯..珍珠粟WD40基因家族鉴定及表达特征分析[J].作物学报,2024,50(9):2219-2236,18.基金项目
本研究由重庆市财政专项资金项目(23515C),重庆市自然科学基金项目(CSTB2022NSCQ-MSX0274),四川省饲草创新团队项目(SCCXTD-2020-16)和四川省育种攻关项目(2021YFYZ0013)资助.This study was supported by the Chongqing Municipal Special Funds Project(23515C),the Chongqing Natural Science Foundation Project(CSTB2022NSCQ-MSX0274),the Sichuan Province Forage Innovation Team Project(SCCXTD-2020-16),and the Sichuan Province Breeding Tackling Project(2021YFYZ0013). （23515C）
