作物学报2024，Vol.50Issue(9)：2237-2247,11.DOI:10.3724/SP.J.1006.2024.34178
过表达马铃薯StuPPO9基因对烟草抗旱能力的影响
Impact of potato StuPPO9 gene overexpression on drought resistance in Nico-tiana benthamiana
摘要
Abstract
To investigate the role of the potato polyphenol oxidase gene StuPPO9 in response to drought stress,we utilized the overexpression of the StuPPO9 gene in Nicotiana benthamiana through Agrobacterium-mediated transformation as the experi-mental material.Pot experiments were conducted to simulate natural drought stress,and various physiological indicators including growth morphology,chlorophyll content,malondialdehyde and proline levels,antioxidant enzyme activity,and expression of re-lated resistance genes were compared and measured in transgenic and wild-type tobacco plants under drought stress for 0,3,6,9 and 12 days.The results revealed that following the drought treatment,the transgenic strains exhibited significantly higher relative water content,chlorophyll content,proline levels,and superoxide dismutase(SOD)and peroxidase(POD)activities compared to the wild-type plants.Conversely,the malondialdehyde content was significantly lower in the transgenic strains compared to the wild-type.RT-qPCR analysis demonstrated a significant upregulation of expression levels in ROS scavenging system genes(SOD,POX),ABA biosynthesis genes(NCED,RD29A),proline biosynthesis gene(P5CS),and other stress response genes(LEA,ADC,SAMDC).These findings suggest that the overexpression of the StuPPO9 gene enhances drought tolerance in tobacco plants,pro-viding a theoretical foundation for further exploration of StuPPO9's role in regulating drought stress response.关键词
StuPPO9/转基因烟草/抗旱性/功能鉴定Key words
StuPPO9/transgenic tobacco/drought tolerance/functional identification引用本文复制引用
刘波,池明,曹梦琦,唐达,杨恒照,张卫华,薛聪..过表达马铃薯StuPPO9基因对烟草抗旱能力的影响[J].作物学报,2024,50(9):2237-2247,11.基金项目
本研究由天津市教委科研计划项目(2018KJ192)资助.This study was supported by the Tianjin Municipal Education Commission(2018KJ192). （2018KJ192）
