作物学报2024，Vol.50Issue(9)：2267-2278,12.DOI:10.3724/SP.J.1006.2024.34163
棉花MNP标记位点开发及其在DNA指纹图谱构建中的应用
Development and application of cotton MNP marker for fingerprint cons-truction
摘要
Abstract
Cotton is an important economic crop with complex genomes,yet the development of cotton molecular markers lags behind that of other diploid crops,posing challenges.This study utilized the highly polymorphic and accurate Multiple Dispersed Nucleotide Polymorphisms(MNP)marker approach to construct fingerprints for the main cotton varieties in China.This achievement provides a technical foundation for variety identification,genetic diversity analysis,and molecular breeding.Using public cotton genome information,a panel of 940 MNP markers was developed.Subsequently,98 cotton samples underwent mul-tiplex PCR amplification,library construction,sequencing,and genotyping.As a result,a fingerprint database for each cotton variety was established.Pairwise comparisons based on MNP genotypes were conducted to determine genetic differences,fol-lowed by a sequential phylogenetic analysis.Effective amplification products were obtained from all 940 MNP loci in the 98 cot-ton samples,with amplification product lengths ranging from 163 to 274 bp and an average of 270 bp.A total of 940 polymorphic loci were detected in the 98 samples,with an average discriminative power(DP)of 0.46.When comparing genetic differences between any two varieties,an average of 410.26 MNP loci showed differences,accounting for an average difference of 44.84%.The NJ phylogenetic tree revealed that the 98 cotton samples could be divided into three groups based on MNP markers,roughly corresponding to their distribution in the Yellow River basin,the Yangtze River basin,and the Xinjiang region.The 940 MNP markers developed in this study can accurately classify cotton varieties,and the DNA fingerprints of the 98 cotton varieties con-structed using these markers provide technical support for variety identification,variety rights protection,and germplasm innova-tion.关键词
棉花/分子标记/MNP/指纹图谱/品种鉴定Key words
cotton/marker/MNP/fingerprint/variety identification引用本文复制引用
艾莎,闫多子,武星廷,彭海,韩瑞玺,周俊飞,李莎,方治伟,李论,李甜甜,高利芬,陈利红,肖华锋,万人静..棉花MNP标记位点开发及其在DNA指纹图谱构建中的应用[J].作物学报,2024,50(9):2267-2278,12.基金项目
本研究由湖北省科技重大项目(2022ABA001),江汉大学一流学科建设重大专项资助计划(2023XKZ024)和三峡工程鱼类资源保护湖北省重点实验室开放课题项目(2021041-ZHX)资助.This study was supported by the Major Science and Technology Projects in Hubei Province(2022ABA001),the Major Special Funding Pro-gram for the Construction of First Class Disciplines at Jianghan University(2023XKZ024),and the Director's Fund of the Hubei Key Labo-ratory of Three Gorges Project for Conservation of Fishes,China Three Gorges Corporation(2021041-ZHX). （2022ABA001）
