作物学报2024，Vol.50Issue(9)：2297-2309,13.DOI:10.3724/SP.J.1006.2024.34193
马铃薯TIFY基因家族的全基因组鉴定及表达分析
Genome-wide identification and expression analysis of TIFY gene family in potato(Solanum tuberosum L.)
摘要
Abstract
The TIFY family is a gene family of transcription factor unique to plants.It contains conserved TIFY domains(TIF[F/Y]XG),which plays an important role in regulating plant development,responding to abiotic stress,and plant hormones.In this study,a total of 26 members of the StTIFY family at the genome-wide level in potatoes(Solanum tuberosum L.)were identified.Based on phylogenetic characteristics and protein structure,StTIFY was divided into 4 subfamilies,and the JAZ subfamily was divided into 5 subgroups.Tandem duplication and segmental duplication events played a major role in the expansion of the StTIFY gene family.Collinearity analysis showed that there were 21,5,and 6 orthologous gene pairs between StTIFYs and TIFYs of dicotyledonous plants,which were tomato(Solanum lycopersicum),Arabidopsis,and cabbage(Brassica oleracea).There were only 1 and 2 orthologous gene pairs between StTIFYs and TIFYs of monocotyledonous rice(Oryza sativa)and maize(Zea mays),respectively,which had evolved under purification selection.RNA-seq data analysis showed that TIFY gene and two genes in ZML subfamily were highly expressed in 13 tissues,while the other members exhibited tissue-specificity.Furthermore,we ana-lyzed the response of the StTIFY gene to abiotic stresses and hormone treatments,and found that 19 genes responded to abiotic stress(drought/salt/heat),among which 3 genes and 2 genes simultaneously responded to 3 and 2 abiotic stresses,respectively.Five genes were differentially expressed under BAP,ABA,IAA,and GA3 treatments.The relative expression levels of four candidate genes in drought-tolerant and water-sensitive cultivars under drought stress were further detected using qPCR.The re-sults showed that these four genes were significantly differentially expressed in the two cultivars during flower-falling period.This study provides a theoretical basis for understanding the characteristics of the StTIFY gene family,as well as in-depth analysis of the functions of StTIFY family members response to hormone signals and resistance to abiotic stress.关键词
马铃薯/TIFY基因家族/激素处理/非生物胁迫/表达分析Key words
potato/TIFY gene family/abiotic stress/hormone treatments/expression analysis引用本文复制引用
祁稼民,许春苗,肖斌..马铃薯TIFY基因家族的全基因组鉴定及表达分析[J].作物学报,2024,50(9):2297-2309,13.基金项目
本研究由定西市科技计划项目(DX2023BZ81)资助.This study was supported by the Science and Technology Program of Dingxi City(DX2023BZ81). （DX2023BZ81）
