作物学报2024，Vol.50Issue(9)：2408-2414,7.DOI:10.3724/SP.J.1006.2024.34204
以甘蓝下胚轴为外植体的再生体系优化
Optimization of regeneration system from hypocotyls of Brassica oleracea L.
摘要
Abstract
Addressing the challenges of a difficult regeneration system and low culture efficiency in the tissue culture process of Brassica oleracea L.,this study focused on using the hypocotyl of Brassica albograbra Bailey as explants,which was identified as a key influencing factor in in vitro culture.A comparative analysis was conducted to assess the effects of light/dark culture time,NaClO disinfection time,and hormone ratio on the differentiation and regeneration of Brassica oleracea L.seeds and adventitious buds.The results showed that the best growth of sterile seedlings was achieved when the seeds were disinfected with 75%anhy-drous ethanol for 40 s+5%NaClO 15 minutes.The seeds were then cultured in the dark for 3 d and then,followed by 3 d in the light.The optimal hormone combination for the pre-culture medium was 0.05 mg L-1 NAA+4 mg L-1 6-BA.In the screening cul-ture medium,the optimal hormone combination was 0.05 mg L-1 NAA+4 mg L-1 6-BA+250 mg L-1 Cb(Carbenicillin)inci-dence of 91.1%and a differentiation rate of adventitious buds of 57.3%.For the rooting medium MS+30 g L-1 sucrose+7 g L-1 agar+0.1 mg L-1 NAA+0.1%activated carbon yielded a large number of adventitious roots with well-developed root systems and strong growth potential.The optimized tissue culture system established in this study for Brassica oleracea L.allows for the rapid production of regenerated plants.This system is beneficial for accelerating the breeding and evaluation of improved varieties of Brassica oleracea L.关键词
白花芥蓝/下胚轴/离体再生/激素Key words
Brassica albograbra Bailey/hypocotyl/in vitro regeneration/hormone combination引用本文复制引用
赵美,刘一玮,石翔天,李亚俐,申树林,尹能文,赵会彦,傅鹰,曲存民..以甘蓝下胚轴为外植体的再生体系优化[J].作物学报,2024,50(9):2408-2414,7.基金项目
本研究由国家自然科学基金项目(32272150)和重庆市自然科学基金项目(CSTB2022NSCQ-LZX0034,CSTB2022TIAD-KPX0010)资助.This study was supported by the National Natural Science Foundation of China(32272150)and the Natural Science Foundation of Chongqing(CSTB2022NSCQ-LZX0034,CSTB2022TIAD-KPX0010). （32272150）
