重庆邮电大学学报（自然科学版）2024，Vol.36Issue(4)：707-715,9.DOI:10.3979/j.issn.1673-825X.202307090224
乳腺癌脑转移差异表达基因的生物学功能分析
Biological function analysis of differentially expressed genes in brain metastasis breast cancer
摘要
Abstract
To explore the pathogenesis of breast cancer brain metastasis(BCBM),the biological functions of differentially expressed genes in BCBM were investigated and the hub genes of BCBM were screened in this study.Four gene expression profiles of BCBM datasets(GSE12237,GSE100534,GSE12598,and GSE43837)were retrieved from the gene expression omnibus(GEO)database and screened by R packages to obtain the differentially expressed genes of BCBM.The functional annotations of differentially expressed genes were conducted by gene ontology(GO)analysis.The enrichment analysis of signal pathways of differentially expressed genes was explored by Kyoto encyclopedia of genes and genomes(KEGG)analy-sis.The protein-protein interaction network was structured by the STRING database to screen the hub genes that might par-ticipate in BCBM.Finally,survival analysis was conducted by Kaplan-Meier survival analysis database.As a result,a total of 261 differentially expressed genes present in 2 or more datasets were screened out.Functional analysis showed that those differentially expressed genes were related to biological processes such as extracellular matrix organization and extracellular structural organization,molecular functions such as extracellular matrix structural composition and collagen binding,and cellular components such as collagen-containing extracellular matrix and collagen trimer.Signal pathways were mainly en-riched in protein digestion and absorption,focal adhesion pathways.Nine hub genes were screened by using protein interac-tion network analysis,among which two hub genes(DCN,COL6A1)were significantly correlated with the survival proba-bility of BCBM.The two hub genes could serve as potential hub genes for BCBM and might provide ideas for molecular mechanism research.关键词
乳腺癌/脑转移/生物信息学/差异表达基因/功能分析/关键调控基因Key words
breast cancer/brain metastasis/bioinformatics/differentially expressed genes/function analysis/hub genes分类
信息技术与安全科学引用本文复制引用
陆博文,秦森彪,邱鑫煜,舒坤贤,浦丹..乳腺癌脑转移差异表达基因的生物学功能分析[J].重庆邮电大学学报（自然科学版）,2024,36(4):707-715,9.基金项目
国家自然科学基金项目(61801071)The National Natural Science Foundation of China(61801071) （61801071）
