南方农业学报2024，Vol.55Issue(6)：1653-1661,9.DOI:10.3969/j.issn.2095-1191.2024.06.010
抗辣椒青枯病的糖类筛选及抗性机制研究
Screening of sugar for resistance to pepper bacterial wilt and its resistance mechanism
摘要
Abstract
[Objective]The aim of this study was to screen sugar for resistance to pepper bacterial wilt and analyze its resistance mechanism,providing a theoretical basis for the development of control agents against pepper bacterial wilt.[Method]Ten sugars of sucrose,maltose,L-arabinose,D-lactose,D-raffinose,D-fructose,D-galactose,D-glucose,D-ribose and D-xylose were used as experimental materials,the tested strain was Ralstonia pseudosolanacearum RS03.Ana-lyzed the assimilation of R.pseudosolanacearum on sugar,seedling experiments and indoor pot experiments were con-ducted to screen for the sugar with the best control effect on pepper bacterial wilt.The interaction between the selected sugar and R.pseudosolanacearum was further studied by the tests of antimicrobial activity,pathogenicity and chemotactic response.And the activities of denfense enzymes such as peroxidase(POD),phenylalanine ammonia-lyase(PAL)and polyphenol oxidase(PPO)activities in the stems of sugar-treated pepper plants were determined at 2,4,6,and 8 d after inoculation with R.pseudosolanacearum.[Result]Among the ten sugars,R.pseudosolanacearum showed negative as-similation to L-arabinose,D-lactose and D-raffinose,therefore they were selected for the follow-up test.The seedling test results indicated that both L-arabinose and D-raffinose significantlyreduced the incidence of pepper bacterial wilt(P＜0.05,the same below).The results of pot experiment showed that L-arabinose had the best control effect on pepper bacte-rial wilt,reaching 90.67％.The interaction analysis between L-arabinose and R.pseudosolanacearum showed that L-arabinose had no inhibitory effect on R.pseudosolanacearum and no effect on its pathogenicity.Additionally,R.pseudo-solanacearum did not show chemotactic response towards L-arabinose.Defense enzyme activity tests further indicated that L-arabinose treatment could significantly increase the activities of POD,PAL,and PPO in pepper stems.The activi-ties of POD and PPO both showed an increasing trend with the extension of inoculation time,reaching their peaks at 571.11 and 125.33 U/g after 8 d of inoculation,respectively.The PAL activity showed a fluctuating trend,reaching its peak at 24.13 U/g on the 4th d of inoculation.[Conclusion]L-arabinose has the best control effect on pepper bacterial wilt.It may enhance the resistance of pepper to bacterial wilt by increasing the activity of defense enzymes within the plant,and has the potential to be developed into a new type of control agent for pepper bacterial wilt.关键词
辣椒/青枯病/糖/筛选/防御酶活性Key words
pepper/bacterial wilt/sugar/screening/defense enzyme activity分类
农业科技引用本文复制引用
高于岚,高连宝,傅慧珍,汪志伟,成善汉,朱国鹏,于文进..抗辣椒青枯病的糖类筛选及抗性机制研究[J].南方农业学报,2024,55(6):1653-1661,9.基金项目
Hainan Natural Science Foundation(322QN255) （322QN255）
Hainan Science and Technology Talent Innova-tion Project(KJRC2023D08) （KJRC2023D08）
Start-up Scientific Research Foundation of Hainan University[KYQD(ZR)-22124] 海南省自然科学基金项目(322QN255) （ZR）
海南省科技人才创新项目(KJRC2023D08) （KJRC2023D08）
海南大学科研启动基金项目[KYQD(ZR)-22124] （ZR）
