南方农业学报2024，Vol.55Issue(6)：1671-1681,11.DOI:10.3969/j.issn.2095-1191.2024.06.012
响应面法优化生防暹罗芽孢杆菌JZ1-4-10的发酵参数
Optimization of fermentation parameters against Bacillus siamensis JZ1-4-10 by response surface method
摘要
Abstract
[Objective]The study aimed to optimize the fermentation culture medium composition formulation and conditions of Bacillus siamensis JZ1-4-10 to improve its antibacterial activity against potato dry rot bacteria,laying a foundation for the subsequent industrial development and agricultural application of related biocontrol agents.[Method]The optimal carbon source,nitrogen source,inorganic salt and suitable concentration of B.siamensis JZ1-4-10 were screened by the single factor test.The optimal fermentation culture medium composition formulation of B.siamensis JZ1-4-10 was obtained using response surface methodology.Based on these findings,the fermentation conditions such as fermentation temperature,pH,inoculation amount,liquid volume and rotational speed of strain JZ1-4-10 were opti-mized.[Result]The optimal carbon source,nitrogen source and inorganic salt of B.siamensis JZ1-4-10 were corn flour,soy peptone and KC1 respectively.With corn flour(A),soy peptone(B)and KC1(C)as the dependent variables,and the antibacterial activity of B.siamensis JZ1-4-10 against the potato dry rot pathogen Fusarium solani as the response value,the quadratic multiple regression equation obtained for the inhibition rate(y)of B.siamensis JZ1-4-10 was y=64.02+0.4937A+2.31B-2.19C-1.17AB+138AC-1.81BC-4.73A2-4.58B2-386C2.The interactions among corn flour,soy peptone and KC1 significantly influenced the antibacterial activity of B.siamensis JZ1-4-10(P＜0.05),especially the interactions between corn flour and KC1 and between soy peptone and KC1 were extremely significant(P＜0.01).The optimum propor-tion of fermentation culture medium composition formulation by response surface analysis for B.siamensis JZ1-4-10 were as follows:0.94％corn flour,1.23％soy protein and 0.72％KC1.Under this condition,the actual value of antibacterial activity of B.siamensis JZ1-4-10 was 65.20％,which was very close to the predicted value of 64.63％.Similarly,the opti-mal fermentation conditions optimized by single factors of B.siamensis JZ1-4-10 were liquid volume of 90 mL/250 mL,inoculation amount of 1.5％,rotational speed of 160 r/min,culture temperature of 30 ℃,and pH of 7.[Conclusion]The optimized culture medium enhances the sporulation and antibacterial activity of B.siamensis JZ1-4-10,the optimized fermentation method can be used to culture the suspension of strain JZ1-4-10 in fast speed and by large amount.关键词
暹罗芽孢杆菌/培养基/培养条件/响应面法/发酵优化Key words
Bacillus siamensis/culture medium/culture conditions/response surface method/fermentation optimi-zation分类
植物保护学引用本文复制引用
蒙耀,陈红雨,吴慧芳,魏琳,沈硕..响应面法优化生防暹罗芽孢杆菌JZ1-4-10的发酵参数[J].南方农业学报,2024,55(6):1671-1681,11.基金项目
National Natural Science Foundation of China(32160659) （32160659）
Qinghai Applied Basic Research Pro-ject(2023-ZJ-702) （2023-ZJ-702）
2022 Qinghai"Kunlun Talents·High-end Innovation and Entrepreneurial Talents"Training Leading Talents Project(2022) 国家自然科学基金项目(32160659) （2022）
青海省应用基础研究项目(2023-ZJ-702) （2023-ZJ-702）
2022年度青海省"昆仑英才·高端创新创业人才"培养领军人才项目(2022) （2022）
