南方农业学报2024，Vol.55Issue(6)：1778-1786,9.DOI:10.3969/j.issn.2095-1191.2024.06.022
宫内发育迟缓猪胎盘SLC7A2基因过表达对滋养层细胞增殖和迁移的影响
Effects of SLC7A2 gene overexpression on trophoblast cell proliferation and migration in the placenta of pigs with intrauterine growth retardation
摘要
Abstract
[Objective]This study aimed to investigate the expression differences of solute carrier family 7 member 2 gene(SCL7A2)in the placentas of normal birth weight(NBW)piglets and intrauterine growth retardation(IUGR)pig-lets,and determine the effect of SCL7A2 gene overexpression on the proliferation and migration of trophoblast cells(pTr2)to provide a reference for revealing the occurrence and development mechanism of IUGR in pigs.[Method]Real-time fluorescence quantitative PCR(RT-qPCR)was used to detect the relative expression of SLC7A2 gene in NBW and IUGR placentas.The localization,distribution and expression level of SLC7A2 protein in placental tissues of NBW and IUGR were analyzed by immunohistochemistry.The effects of SLC7A2 gene overexpression on the proliferation were de-tected by CCK-8 assay,effects of SLC7A2 gene overexpression on migration ability of pTr2 cells were detected by cell scratch assay.[Result]The relative expression of SLC7A2 gene in IUGR placentas was extremely significantly higher(P＜0.01,the same bellow)than that in NBW placentas.SLC7A2 protein was expressed in both trophoblast cells and vascular endothelial cells in pig placentas,with a significantly higher expression level in IUGR placentas compared to NBW pla-centas(P＜0.05,the same bellow).The constructed SLC7A2 gene overexpression vector(Vector-SLC7A2+)was trans-fected into pTr2 cells,resulting in an extremely significant up-regulation of the relative expression of SLC7A2 gene in the cells compared to the pEGFP-C1 negative control vector(Vector-NC)transfected group.However,there was no signifi-cant difference in the relative expression of pEGFP-C1 between the two groups(P＞0.05).After transfection of pTr2 cells with Vector-SLC7A2+and Vector-NC respectively,the proliferation of pTr2 cells in the Vector-SLC7A2+transfected group was significantly or extremely significantly lower than that in the Vector-NC transfected group at 12,24,48,and 72 h post-transfection.Similarly,the migration rate of pTr2 cells in the Vector-SLC7A2+transfected group was extremely sig-nificantly lower than that in the Vector-NC transfected group,demonstrating that SLC7A2 gene overexpression could ef-fectively inhibit proliferation and migration capabilities of pTr2 cells.[Conclusion]Overexpression of the SLC7A2 gene can inhibit the proliferation and migration of porcine placental trophoblast cells,affecting placental morphological develop-ment and maternal-fetal nutrient transport efficiency,and ultimately contributing to the occurrence and development of IUGR in pigs.关键词
猪/SLC7A2基因/胎盘/宫内发育迟缓(IUGR)/营养转运Key words
pigs/SLC7A2 gene/placenta/intrauterine growth retardation(IUGR)/nutrition transportation分类
农业科技引用本文复制引用
胡光玲,伍治敏,张才在,杨振清,张依裕,敖政..宫内发育迟缓猪胎盘SLC7A2基因过表达对滋养层细胞增殖和迁移的影响[J].南方农业学报,2024,55(6):1778-1786,9.基金项目
Guizhou Science and Technology Plan Project(QKHJC-ZK[2021]Yiban 166) （QKHJC-ZK[2021]Yiban 166）
Guizhou Science and Technology Talent Project(QKHPTRC[2021]5630) （QKHPTRC[2021]5630）
Young Science and Technology Talent Development Project in Regular Higher Education Institutions of Guizhou(Qianjiaohe KY[2021]081) （Qianjiaohe KY[2021]081）
Guizhou Pig Industry Development Project(Qiancainong[2021]157) 贵州省科技计划项目(黔科合基础-ZK[2021]一般166) （Qiancainong[2021]157）
贵州省科技人才项目(黔科合平台人才[2021]5630号) （黔科合平台人才[2021]5630号）
贵州省普通高等学校青年科技人才成长项目(黔教合KY字[2021]081号) （黔教合KY字[2021]081号）
贵州省生猪产业发展项目(黔财农[2021]157号) （黔财农[2021]157号）
