南方农业学报2024，Vol.55Issue(6)：1787-1797,11.DOI:10.3969/j.issn.2095-1191.2024.06.023
柔嫩艾美耳球虫感染鸡盲肠内容物非靶向代谢组学分析
Non-targeted metabolomics analysis of cecal contents in chickens infected with Eimeria tenella
摘要
Abstract
[Objective]This study aimed to investigate the differential changes in metabolites in the cecal contents of chickens infected with Eimeria tenella using non-targeted metabolomics techniques,to provide reference for the identifi-cation and determination of potential biomarkers and therapeutic targets for coccidiosis.[Method]Sixty healthy Roman pink chickens were randomly divided into a normal group and a model group,with 30 chickens in each group.The model group was orally administered sporulated oocysts of E.tenella(1×104 oocysts/chicken),while the normal group was orally administered equal volume of normal saline for consecutive 5 d.Then,the experimental chickens were dissected and the chicken cecal contents were aseptically collected.A non-targeted metabolomics analysis was performed on the chicken cecal contents of the normal and model groups to screen for significantly differential metabolites to conduct KEEG metabolic pathway enrichment analysis.[Result]A total of 253 significantly differential metabolites were screened from the chicken cecal contents of the model group and the normal group,of which 184 significantly differential metabo-lites up-regulated expression,including L-tyrosine,L-methionine,L-glutamine,taurine,arachidonic acid,ethyl docosa-hexaenoate,choline and phosphorylcholine.There were 69 significantly differential metabolites down-regulated expres-sion,including deoxyadenosine,L-valine,N-(5-aminoamyl)acetamide and N-acetyl-L-glutamate.In the positive ion mode,20 metabolites,including taurine,phosphorylcholine,betaine,L-leucine,L-glutamine,choline,sphingosine and L-tryptophan,were mainly up-regulated.In the negative ion mode,20 metabolites such as arachidonic acid,taurine,L-phenylalanine,L-leucine,succinic acid,xanthine,uracil and L-glutamine were mainly up-regulated.The significant differential metabolites in chicken cecal contents were mainly enriched in 20 KEEG metabolic pathways,such as ABC transporter,aminoacyl-tRNA biosynthesis,lysine degradation,pyrimidine metabolism,purine metabolism and unsatu-rated fatty acid biosynthesis.[Conclusion]After infection with E.tenella,the metabolites of the chicken cecum change significantly,primarily affecting metabolic pathways such as ABC transporter,aminoacyl-tRNA biosynthesis,amino acid metabolism,pyrimidine metabolism and purine metabolism.Therefore,significant differential metabolites such as taurine,phosphorylcholine,L-glutamine and arachidonic acid can be used as potential biomarkers for the diagnosis of coccidiosis in chickens.关键词
鸡/柔嫩艾美耳球虫/盲肠内容物/差异代谢物/非靶向代谢组学Key words
chicken/Eimeria tenella/cecal contents/differential metabolites/non-targeted metabolomics分类
农业科技引用本文复制引用
翟少钦,闫志强,陈春林,朱买勋,唐红梅,谢三磊,付文贵..柔嫩艾美耳球虫感染鸡盲肠内容物非靶向代谢组学分析[J].南方农业学报,2024,55(6):1787-1797,11.基金项目
Youth Project of National Natural Science Foundation of China(32102633) （32102633）
Chongqing Natural Science Foundation(cstc2019jcyj-msxmX0061) （cstc2019jcyj-msxmX0061）
Sichuan Regional Innovation Cooperation Project(2023YFQ0037) 国家自然科学基金青年基金项目(32102633) （2023YFQ0037）
重庆市自然科学基金项目(cstc2019jcyj-msxmX0061) （cstc2019jcyj-msxmX0061）
四川省区域创新合作项目(2023YFQ0037) （2023YFQ0037）
