加味半夏泻心汤拆方对幽门螺旋杆菌相关性胃炎小鼠的作用及机制研究OA北大核心CSTPCD

Objective:To investigate the effect and mechanism of the disassembled prescriptions of Modified Banxia Xiexin Decoction on Helicobacter pylori-associated gastritis in mice.Methods:Forty SPF-grade male BALB/c mice were randomly di-vided into the blank control group(n=6)and the model group(n=34).The mouse model of Helicobacter pylori associated gastri-tis with damp-heat syndrome of spleen and stomach was established and randomly divided into the Modified Banxia Xiexin Decoc-tion group,Qingrezaoshi group,Jianpihewei group,Zaoshixiaopi group and model group,with 6 mice in each group.Each group was given the corresponding drugs by gavage for 4 weeks.The general biological characteristics of mice were observed and record-ed;enzyme-linked immunosorbent assay(ELISA)was used to detect the expression levels of interleukin-6(IL-6),interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in the sera of mice;hematoxylin-eosin(HE)staining and Giemsa staining were used to observe Hp colonization and pathological changes of the gastric mucosa;quantitative real-time PCR(qPCR)and immuno-histochemistry were used to detect the mRNA and protein expression levels of genes related to the nuclear factor-κB(NF-κB)sig-naling pathway in the gastric mucosa tissues of mice;qPCR was used to detect the contents of Lactobacillus,Bifidobacterium,Enterobacteriaceae,and Enterococcus in the intestinal tracts of mice.Results:Compared with the blank group,the mice in the model group had a decrease in diet and water intake,listlessness,reduced activity,severe gastric mucosal tissue lesions,a large number of Hp colonization,increased serum expression levels of IL-1β,IL-6 and TNF-α(P＜0.01),the mRNA and protein ex-pressions of NF-κB p65 were up-regulated(P＜0.01),while the mRNA and protein expressions of IκB-α were down-regulated(P＜0.01),the contents of Enterobacteriaceae and Enterococcus were increased(P＜0.01),and the contents of Bifidobacterium and Lactobacillus were decreased(P＜0.01);compared with the model group,the general condition and gastric mucosal lesions of the mice in each treatment group were improved to different degrees,the Hp colonization was reduced,the expression levels of IL-1β,IL-6 and TNF-α in serum were decreased(P＜0.05),the mRNA and protein expressions of NF-κB p65 were down-regulated(P＜0.05),and that of IκB-α were up-regulated(P＜0.05),and the contents of Enterobacteriaceae and Enterococcus were decreased(P＜0.05),and in the above indexes,the effect was the best in the whole formula group,and the Qingrezaoshi group was better than the Jianpihewei group and Zaoshixiaopi group in the disassembled group,while the content of Bifidobacterium and Lactobacil-lus was elevated(P＜0.05),and the Jianpihewei group was superior to the Zaoshixiaopigroup and the Qingrezaoshi group.Conclu-sion:The Modified Banxia Xiexin Decoction can inhibit the colonization of Helicobacter pylori in the gastric mucosa and reduce the damage of the gastric mucosa,which may be related to the inhibition of the inflammatory response mediated by the NF-κB sig-naling pathway;and it can promote the growth of intestinal probiotic bacteria while inhibiting the reproduction of harmful bacteria.The Qingrezaoshi group has the best effect in the antibacterial and anti-inflammatory,and the Jianpihewei group plays a major role in regulating the spleen and stomach,and enhancing human immunity.The whole formula is used to play a synergistic effect of XinKai KuJiang GanBu,which fully reflects the treatment principle of"strengthening healthy Qi and eliminating pathogens,treat-ing both symptoms and root causes"in traditional Chinese medicine.