海南医学院学报2024，Vol.30Issue(16)：1220-1229,10.DOI:10.13210/j.cnki.jhmu.20240429.003
基于RNA-Seq探讨清解化攻方干预重症急性胰腺炎大鼠肠屏障损伤的潜在作用机制
RNA-Seq-based exploration of the potential mechanism of action of Qingjie Huagong decoction in intervening intestinal barrier injury in rats with severe acute pancreatitis
摘要
Abstract
Objective:To explore the potential mechanism of action of Qingjie Huagong decoction(QJHGD)in intervening the intestinal barrier damage in rats with severe acute pancreatitis by using high-throughput RNA-Seq.Methods:A SAP model was established by retrograde pancreaticobiliary injection of sodium oxybate,HE was used to observe the histopathological chang-es in the pancreas and ileum of rats in the blank group,model group and traditional Chinese medicine group,and the levels of se-rum amylase,lipase,DAO and endotoxin in rats were detected by ELISA method;the differentially expressed genes were detect-ed in ileal tissues of rats in each group by RNA-seq sequencing,so as to screen the core genes,biological functions and signaling pathways of the QJHGD for intervening in the intestinal barrier damage in rats with severe acute pancreatitis.Results:Compared with the blank group,the pancreas and ileum tissues of rats in the model group showed obvious pathological changes,which were significantly improved by the intervention of QJHGD;the ELISA results suggested that QJHGD could significantly improve the serum amylase,lipase,DAO and endotoxin levels in rats with SAP(P＜0.05).The RNA-Seq results showed that there were 2 623 genes differentiated in the SAP group from the BG group,and 175 genes were significantly down-regulated after QJHGD treatment,mainly Tnip3 and Lpo,while 320 genes were up-regulated,mainly aicda and Tnn,and so on.There were 175 genes whose expression was significantly down-regulated after QJHGD treatment,mainly Tnip3,Lpo,etc.,while 320 genes were up-regulated,mainly aicda,Tnn,etc.,which were involved in the regulation of biological processes such as oxidative stress and intervened in multiple signaling pathways such as TJ,apoptosis,Toll-like receptor,NOD-like receptor,NF-κB and so on,and played a role in the regulation of oxidative stress.Conclusion:QJHGD may inhibit the intestinal barrier injury of severe acute pan-creatitis by regulating key genes such as Tnip3 and aicda,as well as multiple signaling pathways such as TJ,apoptosis,and Toll-like receptor.关键词
RNA-Seq/重症急性胰腺炎/肠屏障损伤/清解化攻方Key words
RNA-Seq/Severe acute pancreatitis/Intestinal barrier injury/Qingjie Huagong decoction分类
医药卫生引用本文复制引用
朱晓东,陈国忠,卢洁,冯敏超,唐曦平,刘锟荣..基于RNA-Seq探讨清解化攻方干预重症急性胰腺炎大鼠肠屏障损伤的潜在作用机制[J].海南医学院学报,2024,30(16):1220-1229,10.基金项目
This study was supported by National Natural Science Foundation of China(82160890) （82160890）
2022 Guangxi University of Traditional Chinese Medicine Doctoral Research Start-up Fund(2022BS029) 国家自然科学基金资助项目(82160890) （2022BS029）
2022年广西中医药大学引进博士科研启动基金项目(2022BS029) （2022BS029）
