海南医学院学报2024，Vol.30Issue(16)：1236-1245,10.DOI:10.13210/j.cnki.jhmu.20240510.001
miR-21-5p靶向BNC2调控食管癌的恶性生物行为的研究
Research on how miR-21-5p targets BNC2 to control the malignant biological activity of esophageal cancer
摘要
Abstract
Objective:To investigate the mechanism by which the identity transcription factor(BNC2)of myoblasts targeted by miR-21-5p in controling the proliferation,migration,and invasion of esophageal cancer(ESCA)cells.Methods:Real-time quantitative polymerase chain reaction(qRT-PCR)was used to assess the relative expression levels of miR-21-5P and BNC2 in esophageal cancer tissues.And any differences in expression were statistically analyzed.Following transfection of ECA109 and KYSE30 esophageal cancer cell lines with miR-21-5p mimics and miR-21-5P inhibitor,and a negative control(NC,including mimcs NC and inhibitor NC),respectively,the cell function test CCK8,and Transwell was used to investigate the impact of miR-21-5p downregulation or overexpression on cell migration,invasion,and proliferation.A bioinformatics technique was uti-lized to identify and investigate the target genes of miR-21-5P.Then,the dual luciferase assay,Western blot,and qRT-PCR as-say were employed to verify the negative regulatory relationship between miR-21-5p and the target genes.Result:In ESCA tumor tissues,it was possible to determine that BNC2 had reduced relative expression levels.and the relative expression levels of miR-21-5p were greater in tumor tissues by qRT-PCR detection.The outcomes of the CCK8 and Transwell studies demonstrated that overexpression of miR-21-5P increased cell invasion,migration,and proliferation.Cell invasion,migration,and proliferation were all decreased by knocking down miR-21-5P.Put differently,miR-21-5P functions as an oncogene in esophageal cancers.The bioinformatics technique predicted BNC2 to be the target gene of miR-21-5P.According to the results of the dual luciferase assay,miR-21-5P was targeted to wild-type BNC2 ′-UTR.Furthermore,miR-21-5p mimics were shown to be able to drastically lower the BNC2 gene level by western blot and qRT-PCR.On the other hand,a miR-21-5p inhibitor may dramatically raise BNC2 ex-pression in esophageal cancer cells.These findings suggest a link of targeting between miR-21-5P and BNC2.Conclusion:In ES-CA,miR-21-5p targets BNC2,and its regulatory role may contribute to cancer proliferation,migration,and invasion.关键词
食管癌/miR-21-5P/BNC2/预后/肿瘤微环境Key words
Tumor microenvironment/BNC2/MiR-21-5P/Esophageal cancer/Prognosis分类
医药卫生引用本文复制引用
邓雨函,阳梦,吴振华,李卉,热则耶·麦麦提祖农,孙晓宏..miR-21-5p靶向BNC2调控食管癌的恶性生物行为的研究[J].海南医学院学报,2024,30(16):1236-1245,10.基金项目
This study was supported by Xinjiang Uygur Autonomous Region Natural Science Foundation General Project(2016D01C3692021D01C397) 新疆维吾尔自治区自然科学基金面上项目(2016D01C369,2021D01C397) （2016D01C3692021D01C397）
