哈尔滨医药2024，Vol.44Issue(4)：1-4,4.DOI:10.3969/j.issn.1001-8131.2024.04.001
黄连素对幽门螺杆菌多重耐药株hefABC基因表达的影响
The Effects of Berberine in the Expression Quantities of Efflux Pump Genes hef'ABC of Helicobacter Pylori of Multi-drug resistance
摘要
Abstract
Objective To study the the role of berberine(BBR)in the regulation of efflux pump genes hefABC in mul-ti-drug resistant(MDR)strains of Helicobacter pylori(Hp).Methods ①Hp clinical isolates were tested the minimum in-hibitory concentration for amoxicillin,levofloxacin,clarithromycin and metronidazole by E-test method.,in which sensitive strains and MDR strains were screened.②The expression quantities diffenrence of efflux pump genes hefABC between MDR group and sensitive group were detected by Real-time fluorescent quantitative polymerase chain reaction(RT-PCR).③The MIC values of berberine against MDR strains were determined by agar two-fold dilution method.After the MDR strains were cultured at 1/2 MIC concentration of berberine in liquid for 5 generations,RT-PCR was used to detect the mRNA expression difference of the efflux pump genes before and after the addition of berberine.Results ①A total of sixty Hp strains were isolated and screened including fifteen sensitive strains and twelve MDR strains.②The expression quantities of hefA、hefB in the MDR group are significantly higher than that in the sensitive group(P<0.05).There are no high expressions of hefC be-tween two groups(P<0.05).③The expression quantities of Hp0605,Hp0606 of MDR group after the effects of berberine re-markably decrease,compared with that before treated with berberine,and the difference has statistical significance.Conclusions①Multi-drug resistance of H.pylori is related to the high expression of efflux pump genes of Hp0605,Hp0606.②Berberine can inhibit drug resistance in some MDR strains by down-regulating the expression of efflux pump genes hefA and hefB.关键词
黄连素/幽门螺杆菌/多重耐药/外排泵基因Key words
berberine/helicobater pylori/multi-drug resistance/efflux pump gene分类
医药卫生引用本文复制引用
周帆,徐小青,彭君伟,曹勤..黄连素对幽门螺杆菌多重耐药株hefABC基因表达的影响[J].哈尔滨医药,2024,44(4):1-4,4.基金项目
国家自然科学基金(青年)项目(81603468),湖州市科技局公益性应用研究项目(2023GYB15) （青年）
