基础医学与临床2024，Vol.44Issue(9)：1223-1228,6.DOI:10.16352/j.issn.1001-6325.2024.09.1223
乳腺癌类器官的培养及鉴定
Culture and characterization of breast cancer organoids
摘要
Abstract
Objective To establish breast cancer organoids for a long time and to characterize their molecular ex-pression and test their sensitivity to chemotherapy drugs.Methods Breast cancer specimens were digested with col-lagenase to release cancer cells for organoid culture.The organoids were characterized by morphology and ER,PR,HER-2,CK expression by technology of histoimmunofluorescence.Among them,three were tested for paclitaxel,doxorubicin and cisplatin sensitivity.Results A total of 44 cases of breast cancer organoids were established,all of which showed dense spherical-like growth and ER,PR,HER-2,CK,matching their clinical counterpart.Breast cancer organoids were sensitive to chemotherapy drugs paclitaxel,doxorubicin and cisplatin.Conclusions Organoid model,as a new in vitro may reproduce the pathophysiology features with heterogeneity.关键词
乳腺癌/类器官/药敏实验Key words
breast cancer/organoid/drug sensitive test分类
医药卫生引用本文复制引用
夏雨佳,杨振丽,代娣,刘玉琴..乳腺癌类器官的培养及鉴定[J].基础医学与临床,2024,44(9):1223-1228,6.基金项目
中国医学科学院医学与健康科技创新工程-重大协同创新项目(2021-I2M-1-053) （2021-I2M-1-053）
