基础医学与临床2024，Vol.44Issue(9)：1236-1242,7.DOI:10.16352/j.issn.1001-6325.2024.09.1236
流体剪切力通过激活Piezo 1蛋白促进肾小球内皮细胞凋亡
Fluid shear promotes apoptosis of glomerular endothelial cells by activation of Piezo 1
摘要
Abstract
Objective To investigate the effect of fluid shear(FS)on apoptosis of glomerular epithelial cells(GECs)and the role of Piezo 1 protein in it.Methods GECs(glomerular epithelial cells)of SD rat were cul-tured.Fluid shear stimulation was simulated by a Flexcell-T5000 tensiometer.Apoptosis level was detected by flow cytometry.The expression of Piezo 1 proteins in GECs was detected by immunofluorescence staining.The activating of Piezo 1 channels by fluid shear was observed using Ca2+indicator(Cal-590 AM).The effect of Piezo 1 on apop-tosis in GECs was analyzed after modulating the function or expression of Piezo 1 protein using the chemical activa-tor Yoda1,the inhibitor GsMtx 4 was regulated by lentivirus Lv-shPiezo 1.Results Compared with the blank controlgroup,apoptosis increased in the fluid shear group(P＜0.05).The rate of apoptosis increased with the enhancing of fluid shear strength;Piezo 1 was commonly expressed in GECs.Fluid shear activated Piezo 1 chan-nel and enhanced expression of Piezo 1.The agonist Yoda1 promoted the apoptosis of GECs GsMtx 4 inhibited the apoptosis induced by fluid shear.Lv-shPiezo 1 knocked down the expression of Piezo 1 in GECs and the apoptosis rate of GECs in the knockdown group was reduced as compared to that in the control group and Lv-Ctrl group(P＜0.05).Conclusions Fluid shear may promote apoptosis of GECs by activation of Piezo 1 and by enhancing expression of Piezo 1.关键词
流体剪切力/Piezo 1/肾小球内皮细胞/凋亡/Yoda 1Key words
fluid shear/Piezo 1/glomerular epithelial cell/apoptosis/Yoda 1分类
医药卫生引用本文复制引用
罗梦琳,郑峰,季欣瑶,祁彩虹,肖雨珩,牛长春..流体剪切力通过激活Piezo 1蛋白促进肾小球内皮细胞凋亡[J].基础医学与临床,2024,44(9):1236-1242,7.基金项目
青海省卫生健康委员会重点课题(2022-wjzd-02) （2022-wjzd-02）
