检验医学与临床2024，Vol.21Issue(16)：2328-2333,6.DOI:10.3969/j.issn.1672-9455.2024.16.006
白细胞介素-6对胰腺癌小鼠癌组织的细胞增殖及上皮间质转化的影响
Influence of interleukin-6 in cell proliferation and epithelial mesenchymal transition in carcinoma tissues in mice with pancreatic cancer
摘要
Abstract
Objective To explore the role of interleukin-6(IL-6)in the proliferation and epithelial mesen-chymalization of cancerous tissues in a nude mouse model of pancreatic cancer,and to analyze its molecular mechanism.Methods Eighteen BALB/c nude mice,who were successfully constructed with pancreatic cancer cell-loaded tumors,were randomly divided into model control group,IL-6 overexpression group and immuno-globulin G(IgG)antibody group,six mice in each group.The control group was injected with saline at the tumor site for intervention,the IL-6 overexpression group was injected with IL-6 overexpression plasmid at the tumor site for intervention and the IgG antibody group was injected with IgG antibody at the tumor site for intervention.The mice in the three groups were executed 24 h after the last intervention,and the pancreatic tumor tissues were collected.The tumor tissues of each group were weighed ex vivo and the tumor inhibition rate was calculated.The pathological changes of pancreatic tissues were observed by hematoxylin-eosin(HE)staining.The expression levels of IL-6,p-STAT3,NF-κB p65 and VEGF proteins in the tumor tissues of nude mice in each group were detected by enzyme-linked immunosorbent assay(ELISA).The expression levels of E-cadherin,Vimentin,and MMP-9 proteins in tumor tissues of each group were detected by Western blot.The expression levels of IL-6,p-STAT 3 and SOCS3 genes in each group were detected by qRT-PCR.Results Comparison of the average tumor weight and tumor inhibition rate of pancreatic tumor tissues in the three groups of nude mice showed no statistically significant difference(P＞0.05).HE staining results showed that the tumor tissues were with an increase in the cell volume and the cancer cells were arranged in a nested shape in the model con-trol group;tumor tissues could be seen to be mildly fibrotic,with a small amount of inflammatory cell infiltra-tion in the IL-6 overexpression group;nuclear schizophrenia was seen,with a large number of tumor cells nec-rotic and focal tissue fibrosis in the IgG antibody group.In the IL-6 overexpression group,the expression lev-els of E-cadherin protein and SOCS3 gene were lower than those in the model control group and the IgG anti-body group,while the expression levels of Vimentin,MMP-9,IL-6,p-STAT3,NF-κB p65 and VEGF proteins,as well as IL-6 and NF-κB p65 genes,were higher than those in the model control group and the IgG antibody group,and the differences were statistically significant(P＜0.05);in the IgG antibody group,the expression levels of Vimentin,MMP-9,IL-6,p-STAT3,NF-κB p65,VEGF proteins and the expression levels of IL-6 and NF-κB p65 genes were lower than those in the model control group,while the levels of E-cadherin and SOCS3 genes were higher than those in the model control group,and the differences were all statistically different(P＜0.05).Conclusion IL-6 can regulate related cytokines in tumor tissues of nude mouse model of pancreatic cancer through p-STAT3 and/or NF-κB p65 signaling pathway,affecting proliferation and epithelial mesen-chymal transition of tumor tissues.关键词
白细胞介素-6/胰腺癌/裸鼠模型/增殖/上皮间质化/信号通路Key words
interleukin-6/pancreatic cancer/nude mouse model/proliferation/metastasis/signa-ling pathway分类
临床医学引用本文复制引用
崔彦杰,宋金萍,郝立君,黄国虹..白细胞介素-6对胰腺癌小鼠癌组织的细胞增殖及上皮间质转化的影响[J].检验医学与临床,2024,21(16):2328-2333,6.基金项目
新疆维吾尔自治区自然科学基金面上项目(2021D01C190) （2021D01C190）
新疆维吾尔自治区人民医院科技引进创新项目(20190202). （20190202）
