检验医学与临床2024，Vol.21Issue(16)：2362-2366,5.DOI:10.3969/j.issn.1672-9455.2024.16.012
成人癫痫患者血清miR-146a、miR-125a、TLR4水平及临床意义
Levels of serum miR-146a,miR-125a and TLR4 in adult patients with epilepsy and their clinical significance
摘要
Abstract
Objective To explore levels of microRNA(miR)-146a,miR-125a and Toll-like receptor 4(TLR4)in peripheral blood and their relationship with disease severity in adult patients with epilepsy.Meth-ods A total of 69 patients admitted to the People's Hospital of Xinjiang Uygur Autonomous Region from August 2020 to August 2023 were selected as the epilepsy group,and another 69 healthy people who had a physical examination during the same period were selected as the control group.Real-time fluorescence quanti-tative polymerase chain reaction was used to measure serum miR-146a and miR-125a levels,and enzyme-linked immunosorbent assay was used to detect serum TLR4,interleukin(IL)-18,IL-2 and tumor necrosis factor-a(TNF-a)levels in all research objects.Based on the results of EEG detection,epilepsy patients were divided into mild group,moderate group and severe group,and miR-146a,miR-125a,TLR4,IL-1β,IL-2 and TNF-α levels were compared among the three groups.Pearson correlation was used to analyze the relationship between miR-146a,miR-125a and TLR4 levels and IL-1β,IL-2,TNF-α levels,and Spearman correlation was used to analyze the relationship between miR-146a,miR-125a and TLR4 levels and severity of disease.Results The serum miR-146a and miR-125a levels in the epilepsy group were lower than those in the control group,and the levels of TLR4,IL-1β,IL-2 and TNF-α were higher than those in the control group,with statis-tically significant differences(P＜0.05).The results of EEG showed that there were 23,25 and 21 cases in the mild group,moderate group and severe group,respectively,and the levels of serum miR-146a,miR-125a,TLR4,IL-1β,IL-2 and TNF-α among the three groups had statistically significant differences(P＜0.05);the levels of miR-146a and miR-125a in the mild group＞the moderate group＞severe group,while the levels of TLR4,IL-1β,IL-2,and TNF-α in the mild group＜moderate group＜severe group,and comparisons between any two groups had statistically significant differences(P＜0.05).Pearson correlation analysis showed that miR-146a and miR-125a levels were negatively correlated with IL-1β,IL-2 and TNF-α levels(P＜0.05),while TLR4 level was positively correlated with IL-1β,IL-2 and TNF-α levels(P＜0.05).Spearman correlation a-nalysis showed that miR-146a and miR-125a levels were negatively correlated with disease severity(P＜0.05),while TLR4 level was positively correlated with disease severity(P＜0.05).Conclusion The serum miR-146a and miR-125a levels are down-regulated,while TLR4 level is up-regulated in adult patients with epi-lepsy,and the above three indexes are correlated with IL-1β,IL-2 and TNF-α levels,so they can be applied to evaluate disease severity in epilepsy patients.关键词
成人癫痫/微小核糖核酸-146a/微小核糖核酸-125a/Toll样受体4/疾病严重程度/炎症因子/相关性Key words
adult epilepsy/microRNA-146a/microRNA-125a/Toll-like receptor 4/disease severi-ty/inflammatory factor/correlation分类
医药卫生引用本文复制引用
仲婷,陆明佳,米尔古丽·艾麦特,焦燕,李红燕..成人癫痫患者血清miR-146a、miR-125a、TLR4水平及临床意义[J].检验医学与临床,2024,21(16):2362-2366,5.基金项目
新疆维吾尔自治区自然科学基金资助项目(2019D01C149). （2019D01C149）
