检验医学与临床2024，Vol.21Issue(16)：2390-2395,6.DOI:10.3969/j.issn.1672-9455.2024.16.017
皮肤鳞状细胞癌组织miR-20a、miR-196a表达水平与临床病理特征和预后的关系
Relationship of miR-20a,miR-196a expression levels in cutaneous squamous cell carcinoma tissues with clinicopathological features and prognosis
摘要
Abstract
Objective To investigate the relationship of microRNA(miR)-20a,miR-196a expression levels in cutaneous squamous cell carcinoma(CSCC)tissues with clinicopathological features and prognosis.Methods A total of 316 CSCC patients who underwent surgical resection and successfully followed up in the hospital from January 2014 to January 2018 were selected as the study subjects.The miR-20a and miR196a expression levels in cancer tissues and cancer adjacent tissues(distance from cancer tissues＞3 cm)of CSCC patients were detected by real-time fluorescence quantitative polymerase chain reaction.The relationship be-tween the expression levels of miR-20a and miR-196a and the clinicopathological features of CSCC was ana-lyzed.The influencing factors affecting the poor prognosis of CSCC patients were analyzed by multivariate Lo-gistic regression analysis.According to the mean expression level of miR-20a and miR-196a in CSCC tissues,CSCC patients were divided into miR-20a high expression group,miR-20a low expression group and miR-196a high expression group,miR-196a low expression group.The 5-year survival differences of miR-20a high ex-pression group,miR-20a low expression group and miR-196a high expression group,miR-196a low expression group were analyzed by drawing Kaplan-Meier curve.Results Compared with cancer adjacent tissues,the ex-pression level of miR-20a in cancer tissues of CSCC patients was decreased,and the expression level of miR-196a was increased,with statistically significant differences(P＜0.05).The differences on expression levels of miR-20a and miR-196a were statistically significant in CSCC patients with different degree of differentiation,TNM stage and whether lymph node metastasis occurred or not(P＜0.05).At 5-year follow-up,58 CSCC pa-tients died(death group)and 258 cases survived(survival group),with a 5-year overall survival rate of 81.65％(258/316).The proportion of patients with low differentiation,the proportion of patients with TNM stage Ⅲ,the proportion of patients with lymph node metastasis and the expression level of miR-196a in cancer tissues in the death group were higher than those in the survival group,while the expression level of miR-20a in cancer tissues was lower than that in the survival group,and the differences were all statistically significant(P＜0.05).Multivariate Logistic regression analysis showed that low differentiation,TNM stage Ⅲ,lymph node metastasis and miR-1 96a＞0.94 were independent risk factors affecting the poor prognosis of CSCC pa-tients(P＜0.05),and miR-20a＞0.80 was an independent protective factor(P＜0.05).Kaplan-Meier survival curves results showed that the survival curve of patients in high miR-20a expression group in cancer tissues was higher than that of patients in low miR-20a expression group(Log-rank x2=4.152,P=0.042),and the survival curve of patients in high miR-196a expression group in cancer tissues was lower than that of patients in low miR-196a expression group(Log-rank x2=4.879,P=0.027).Conclusion Low expression of miR-20a and the high expression of miR-196a in CSCC tissues are related to the degree of differentiation,TNM stage,lymph node metastasis and poor prognosis,which may become new biomarkers for predicting the prognosis of CSCC patients.关键词
皮肤鳞状细胞癌/微小RNA-20a/微小RNA-196a/临床病理特征/预后Key words
cutaneous squamous cell carcinoma/microRNA-20a/microRNA-196a/clinicopathologi-cal features/prognosis分类
医药卫生引用本文复制引用
姚佳雯,时永强..皮肤鳞状细胞癌组织miR-20a、miR-196a表达水平与临床病理特征和预后的关系[J].检验医学与临床,2024,21(16):2390-2395,6.基金项目
陕西省2021年科技计划项目(2021JQ-609). （2021JQ-609）
