检验医学与临床2024，Vol.21Issue(16)：2417-2421,2426,6.DOI:10.3969/j.issn.1672-9455.2024.16.022
术前血清sTim-3、HMGB1水平与肌层浸润性膀胱癌根治术后预后的关系
Relationship between preoperative serum sTim-3,HMGB1 levels and the prognosis of muscle-invasive bladder cancer after radical surgery
摘要
Abstract
Objective To investigate the relationship between serum soluble T cell immunoglobulin domain and mucin domain-3(sTim-3)and high mobility group box 1 protein(HMGB1)levels before surgery and the prognosis of muscle-invasive bladder cancer(MIBC)after radical surgery.Methods Eighty-five MIBC pa-tients admitted to the hospital from June 2019 to June 2020 were selected as the MIBC group,and another 85 healthy people who had a physical examination in the hospital during the same period were selected as the con-trol group.Enzyme-linked immunosorbent assay(ELISA)was used to detect serum sTim-3 and HMGB1 lev-els in all subjects.MIBC patients were divided into sTim-3 high-expression group(≥mean value)and sTim-3 low-expression group(＜mean value),HMGB1 high-expression group(≥mean value)and HMGB1 low-ex-pression group(＜mean value)according to the mean values of serum sTim-3 and HMGB1 levels.Serum sTim-3 and HMGB1 levels were compared between different groups,as well as the relationship between dif-ferent serum sTim-3 and HMGB1 levels and clinicopathologic features of MIBC patients.Pearson correlation analysis was used to analyze the correlation between serum sTim-3 level and serum HMGB1 level in MIBC pa-tients.Kaplan-Meier survival curves were used to analyze the survival prognosis of MIBC patients with differ-ent serum sTim-3 and HMGB1 levels.Results The serum sTim-3 and HMGB1 levels in the MIBC group were higher than those in the control group(P＜0.05).The results of Pearson correlation analysis showed a positive correlation between the serum sTim-3 level and the serum HMGB1 level of patients in the MIBC group(r=0.405,P＜0.001).Comparison of serum sTim-3 and HMGB1 levels in MIBC patients with differ-ent ages,genders,maximum tumor diameters and whether lymph node metastasis occurred or not showed no statistically significant differences(P＞0.05),while comparison of serum sTim-3 and HMGB1 levels in MIBC patients with different TNM stages,tissue grades and lymph node status showed statistically significant differ-ences(P＜0.05).Based on the mean values of sTim-3 and HMGB1 levels,85 MIBC patients were divided into the sTim-3 high-expression group(≥3.67 ng/mL,n=44)and sTim-3 low-expression group(＜3.67 ng/mL,n=41),the HMGB1 high-expression group(≥14.91 ng/mL,n=47)and the HMGB1 low-expression group(＜14.91 ng/mL,n=38).Kaplan-Meier survival curves results showed that the 3-year survival curve of pa-tients in the sTim-3 low-expression group was higher than that in the sTim-3 high-expression group(Log-rank x2=6.175,P=0.013),and the 3-year survival curve of patients in the HMGB1 low-expression group was higher than that in the HMGB1 high-expression group(Log-Rank x2=4.056,P=0.044).The 3-year survival rates of MIBC patients in the sTim-3 high-expression group and sTim-3 low-expression group were 52.27％and 78.05％,respectively,while the 3-year survival rates of MIBC patients in the HMGB1 high-ex-pression group and HMGB1 low-expression group were 55.32％and 76.31％,respectively.Conclusion Ser-um sTim-3 and HMGB1 are highly expressed in MIBC patients,and there is a positive correlation between sTim-3 level and HMGB1 level,which can be important indicators for poor prognosis in MIBC patients.关键词
肌层浸润性膀胱癌/可溶性T细胞免疫球蛋白黏蛋白分子-3/高迁移率族蛋白B1/相关性/预后Key words
muscle-invasive bladder cancer/soluble T cell immunoglobulin domain and mucin domain-3/high mobility group box 1 protein/correlation/prognosis分类
医药卫生引用本文复制引用
薛慧英,李鸿斌,赵少成..术前血清sTim-3、HMGB1水平与肌层浸润性膀胱癌根治术后预后的关系[J].检验医学与临床,2024,21(16):2417-2421,2426,6.基金项目
山西省大同市重点研发计划项目(2019012). （2019012）
