精准医学杂志2024，Vol.39Issue(4)：283-288,6.DOI:10.13362/j.jpmed.202404001
顺铂联合衣霉素对神经母细胞瘤的抑制作用及其机制
The inhibitory effect of cisplatin combined with tunicamycin on neuroblastoma and related mecha-nism
摘要
Abstract
Objective To investigate the inhibitory effect of cisplatin(DDP)combined with tunicamycin(TM)on human neuroblastoma SH-SY5Y and SK-N-SH cells and related mechanism.Methods Human neuroblastoma SH-SY5Y and SK-N-SH cells were divided into control group,TM group(treated with 0.4 mg/L TM for 24 h),DDP group(treated with 4 mg/L DDP for 24 h),and DDP+TM group(treated with 0.4 mg/L TM and 4 mg/L DDP for 24 h).CCK-8 assay,plate colony formation assay,scratch assay,and Transwell assay were used to measure the viability and proliferation,migration,and invasion abilities of cells in each group,and Western blotting was used to measure the expression levels of the JAK2-STAT3-HIF1α pathway-related proteins such as JAK-2,p-JAK2,STAT3,p-STAT3,and HIF1α in each group of cells.Results Experimental results showed that com-pared with the other three groups,the DDP+TM group had significantly lower viability,proliferation and invasion abilities,mi-gration ability at 12 and 24 h,and expression levels of the J AK2-STAT3-HIF1α pathway-related proteins(tLSD=2.14-78.95,P＜0.05).Conclusion DDP combined with TM can inhibit the proliferation and migration of neuroblastoma via the JAK2-STAT3-HIF1α pathway,which provides new ideas for the treatment of neuroblastoma.关键词
神经母细胞瘤/顺铂/衣霉素/Janus激酶2/STAT3转录因子/缺氧诱导因子1,α亚基/细胞运动/细胞增殖Key words
Neuroblastoma/Cisplatin/Tunicamycin/Janus kinase 2/STAT3 transcription factor/Hypoxia-inducible factor 1,alpha subunit/Cell movement/Cell proliferation分类
医药卫生引用本文复制引用
张腾龙,孙文静,宋军莹,侯琳..顺铂联合衣霉素对神经母细胞瘤的抑制作用及其机制[J].精准医学杂志,2024,39(4):283-288,6.基金项目
国家自然科学基金资助项目(81472542) （81472542）
山东省自然科学基金资助项目(ZR2023MH122) （ZR2023MH122）
