精准医学杂志2024，Vol.39Issue(4)：309-313,319,6.DOI:10.13362/j.jpmed.202404006
二氢杨梅素对铁过载导致的海马神经元细胞损伤的保护作用及其机制
Protective effect of dihydromyricetin against hippocampal neuronal injury caused by iron overload and its mechanism
摘要
Abstract
Objective To investigate the protective effect of dihydromyricetin(DMY)against injury of primary hip-pocampal neurons caused by ferric ammonium citrate(FAC)and its mechanism.Methods Primary hippocampal neurons were collected from 24-hour neonatal Sprague-Dawley rats for in vitro culture,and CCK-8 assay was used to measure the viability of pri-mary hippocampal neurons treated with different concentrations of DMY and determine the administration concentration of DMY.Primary hippocampal neurons were divided into control group(H group),100 mmol/L DMY treatment group(I group),250 mmol/L FAC treatment group(J group),and 100 mmol/L DMY+250 mmol/L FAC treatment group(K group).Flow cy-tometry was used to measure the content of reactive oxygen species(ROS)in each group;colorimetry was used to measure the content of malondialdehyde(MDA)in each group;Western blot was used to measure the protein expression levels of nuclear factor erythroid 2-related factor 2(Nrf2),heme oxygenase-1(HO-1),and glutathione peroxidase 4(GPX4)in each group.Results CCK-8 assay showed the highest viability of primary hippocampal neurons at the concentration of 100 mmol/L for DMY(F=9.95,P＜0.05),and therefore,this concentration was used for subsequent experiments.Compared with the H group,the J group had significant increases in the content of ROS and MDA(F=176.81,5 523.35,P＜0.05),and compared with the J group,the K group had significant reductions in the content of ROS and MDA(F=18.21,412.96,P＜0.05).Compared with the H group,the J group had significant reductions in the relative protein expression levels of Nrf2,HO-1,and GPX4(F=27.35-81.32,P＜0.05),and compared with the J group,the K group had significant increases in the relative protein expression levels of Nrf2,HO-1,and GPX4(F=8.74-21.46,P＜0.05).Conclusion DMY can alleviate oxidative stress damage in primary hippocampal neurons due to iron overload,possibly by activating the Nrf2-HO-1/GPX 4 pathway in primary hippocampal neurons.关键词
二氢杨梅素/铁超负荷/海马/神经元/氧化性应激/NF-E2相关因子2/血红素加氧酶-1/谷胱甘肽过氧化酶Key words
Dihydromyricetin/Iron overload/Hippocampus/Neurons/Oxidative stress/NF-E2-related factor 2/Heme oxygenase-1/Glutathione peroxidase分类
医药卫生引用本文复制引用
隋云杰,马泽刚..二氢杨梅素对铁过载导致的海马神经元细胞损伤的保护作用及其机制[J].精准医学杂志,2024,39(4):309-313,319,6.基金项目
山东省自然科学基金项目(ZR2022MH018) （ZR2022MH018）
