精准医学杂志2024，Vol.39Issue(4)：320-323,327,5.DOI:10.13362/j.jpmed.202404008
血小板衍生生长因子对脉络膜黑色素瘤细胞活性及侵袭能力的影响
Effect of platelet-derived growth factor on the activity and invasion ability of choroidal melanoma cells
摘要
Abstract
Objective To investigate the effect of platelet-derived growth factor(PDGF)on the activity and invasion ability of choroidal melanoma(CM)cells.Methods CM cells were divided into groups A and B and were transfected with empty vector and PDGF knockdown lentivirus,respectively.RT-qPCR was used to measure the relative expression level of PDGF mR-NA;XTT assay was used to measure cell activity;Transwell assay was used to test the invasion ability of cells in the two groups;Western blot was used to measure the relative expression levels of epithelial-mesenchymal transition(EMT)marker proteins and matrix metalloproteinases(MMPs).GEPIA database was used to investigate the association between PDGF and the prognosis of CM patients.Results Compared with group A,group B had significant reductions in the relative expression level of PDGF RNA(t=176.30,P＜0.05),the number of cells which survived(F=57.21,P＜0.05),and the invasion ability of cells(F=57.21,t=14.10,P＜0.05).Western blot showed that compared with group A,group B had a significant increase in the relative ex-pression level of E-cadherin and significant reductions in the relative expression levels of N-cadherin,Snail,Vimentin,MMP9,and MT1 MMP(t=4.13-14.14,P＜0.05).The GEPIA database analysis showed that the high expression levels of PDGF-A and PDGF-B were associated with the poor prognosis of CM patients(P＜0.05).Conclusion PDGF can enhance the activity of CM cells and promote the invasion of CM cells,possibly by inducing EMT.关键词
黑色素瘤/脉络膜肿瘤/血小板源性生长因子/细胞增殖/肿瘤浸润/上皮-间质转化/基质金属蛋白酶类Key words
Melanoma/Choroid neoplasms/Platelet-derived growth factor/Cell proliferation/Neoplasm invasiveness/Epithelial-mesenchymal transition/Matrix metalloproteinases分类
医药卫生引用本文复制引用
姚宁宁,袁莹莹,马清悦,易雯丹,隋爱华,罗文娟..血小板衍生生长因子对脉络膜黑色素瘤细胞活性及侵袭能力的影响[J].精准医学杂志,2024,39(4):320-323,327,5.基金项目
山东省自然科学基金面上项目(82274585) （82274585）
