精准医学杂志2024，Vol.39Issue(5)：408-412,5.DOI:10.13362/j.jpmed.202405008
银杏内酯B对脂肪肝小鼠肝脏缺血再灌注损伤的影响及其机制
Effect of ginkgolide B on hepatic ischemia-reperfusion injury in mice with fatty liver disease and its mechanism
摘要
Abstract
Objective To investigate the effect of ginkgolide B on hepatic ischemia-reperfusion injury in mice with fatty liver disease and its mechanism.Methods A total of 25 male C57BL/6J mice were fed with 60％high-fat diet for 16 weeks,and then they were divided into sham-operation group(laparotomy and suture),model group(occlusion of blood flow in the middle and left lobes of the liver for 1 h,followed by reperfusion for 6 h),ginkgolide B group(intraperitoneal injection of 25 mg/kg ginkgolide B twice,followed by the treatment in the model group),GW9662 group(intraperitoneal injection of 1 mg/kg GW9662 twice,fol-lowed by the treatment in the model group),and GW9662+ginkgolide B group(intraperitoneal injection of 25 mg/kg ginkgolide B+l mg/kg GW9662 twice,followed by the treatment in the model group).At 6 h after administration and surgery,the serum levels of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)were measured for each group;Western blotting was used to measure the protein expression levels of Bax,Bcl-2,and PPARγ in liver tissue;oil red O staining was used to measure the degree of fatty changes in the liver,and HE staining was used to measure the degree of necrosis in liver tissue.Results Oil red O staining showed that the degree of fatty changes in the liver had reached the level of fatty liver disease in each group.The se-rum enzymatic analysis showed that the ginkgolide B group and the GW9662+ginkgolide B group had significantly lower serum le-vels of ALT and AST than the model group(P＜0.05),and the ginkgolide B group had a significantly lower serum level of ALT than the GW9662+ginkgolide B group(P＜0.05).HE staining showed that the ginkgolide B group had a significantly lower per-centage of necrotic area in liver tissue than the model group and the GW9662+ginkgolide B group(P＜0.05).Western blotting showed that the ginkgolide B group had a significantly lower expression level of Bax in liver tissue than the model group(P＜0.05),as well as significantly higher expression levels of Bcl-2 and PPARy than the model group and the GW9662+ginkgolide B group(P＜0.05).Conclusion Ginkgolide B can alleviate ischemia-reperfusion injury in mice with fatty liver disease by upregu-lating PPARγ in liver tissue.关键词
再灌注损伤/脂肪肝/银杏内酯类/PPARγ/疾病模型,动物Key words
Reperfusion injury/Fatty liver/Bilobalides/PPAR gamma/Disease models,animal分类
医药卫生引用本文复制引用
黄希健,赵金鑫,罗礼键,刘欢,蔡金贞..银杏内酯B对脂肪肝小鼠肝脏缺血再灌注损伤的影响及其机制[J].精准医学杂志,2024,39(5):408-412,5.基金项目
国家自然科学基金项目(82370666) （82370666）
福建省自然科学基金项目(ZR202111260267) （ZR202111260267）
