农业生物技术学报2024，Vol.32Issue(9)：1959-1970,12.DOI:10.3969/j.issn.1674-7968.2024.09.001
一年生野生稻染色体片段代换系的构建及株高和分蘖的QTLs鉴定
Construction of Chromosome Fragment Replacement Lines and QTLs Identification of Plant Height and Tiller in Annual Wild Rice(Oryza nivara)
摘要
Abstract
Wild rice has nearly 1 times more alleles than cultivated rice(Oryza sativa),so it is of great significance to improve rice yield and quality by using wild rice excellent genes.In this study,a set of rice chromosome fragment replacement lines were constructed using annual wild rice(Oryza nivara)as donor parent and indica hybrid rice'Fuhui 676'('FH676')as recipient parent.After one hybridization,three backcrosses,and multiple generations of self-cross,502 BC3F7 individuals were selected for whole genome detection using rice high density whole genome SNP chip.Finally,474 lines containing the genome fragments of the donor parents were used as the establishment population.The results showed that the homozygosity of the whole line was high,and the number of SNP markers in the genetic map was up to 1 429,and the average number of SNP markers in each chromosome was 119.Seventy-two imported lines containing≤4 fragments were selected as the coverage map of the whole genome,and the imported fragments basically covered the whole genome.The total length of target segment substitution in overlay map substitution line population was 581.84 Mb.The total length of the substitution fragment in the whole genome was 306.56 Mb,covering 82.75%of the genome.Plant height and effective tiller spike of the whole line were investigated in Sanya,Hainan province and Youxi,Fujian province.Combined with genotype,QTL analysis of plant height and effective tiller spike was performed by using complete interval mapping.A total of 9 QTLs affecting plant height were detected in the 2 environments,distributed on chromosomes 2,3,7,8 and 11.Only qPH-3 on chromosome 3 was detected in both environments;A QTL qPH-8-1 significantly correlated with plant height was identified,with LOD value of 16.79 and phenotypic contribution rate of 9.16%.Fourteen effective tiller QTLs were detected,distributed on chromosomes 2,3,4,5,6 and 7,and qETN-7-3,qETN-7-4 and qETN-7-8 were detected in both environments.A QTL,qETN-7-6,with a LOD value of 58.28 and a phenotypic contribution rate of 32.86%,significantly correlated with the number of effective tillers was identified.This study provides the theoretical basis for fine QTL localization of plant height and tiller.关键词
一年生野生稻/'福恢676'/染色体片段代换系/株高/有效分蘖数Key words
Annual wild rice/'Fuhui 676'/Chromosome fragment substitution line/Plant height/Effective tiller number分类
农业科技引用本文复制引用
蒋家焕,蔡秋华,魏毅东,刘峰,陈丽萍,朱永生,张建福..一年生野生稻染色体片段代换系的构建及株高和分蘖的QTLs鉴定[J].农业生物技术学报,2024,32(9):1959-1970,12.基金项目
福建省属公益类科研专项(2021RR1023006) （2021RR1023006）
福建省科技重大专项(2022NZ030014) （2022NZ030014）
福建省人民政府-中国农业科学院协同创新工程"5511"项目(XTCXGC2021019) （XTCXGC2021019）
中央引导地方科技发展专项(2022L3018) （2022L3018）
福建省财政专项-福建省农业科学院科技创新团队(CXTD2021005-3) （CXTD2021005-3）
