'新郁'葡萄microRNA的鉴定及其高温胁迫响应分析OA北大核心CSTPCD

High temperature stress is one of the primary abiotic stresses that restrict fruit tree production.Grape(Vitis vinifera)is a cultivated fruit crop with high economic value worldwide,and its growth and development are often affected by high temperature stress.Studying the response mechanism of grapes to high temperature stress is of great significance for understanding the domestication of grapes to high temperature stress.In this study,'Xinyu'grape leaves were collected for high-throughput sequencing and small RNA(sRNA)libraries were constructed for the control group and the high-temperature stress treatment group,respectively.A series of miRNAs related to high temperature stress response were identified,and their functions in high temperature tolerance response were analyzed.A total of 171 known miRNAs and 251 new miRNAs were identified,among which 6 known miRNAs and 21 new identified miRNAs were differentially expressed under high temperature stress.A total of 297 target genes were predicted for these differentially expressed miRNAs.The expression of 10 selected miRNAs and their target genes was validated through qRT-PCR,indicating that these target genes had a significant response to high temperature stress.Gene function and pathway analysis indicated that these genes might play an important role in high temperature stress tolerance.The results of this study provide a theoretical basis for further understanding the high-temperature stress response during fruit tree domestication and the breeding of grape varieties resistant to high-temperature stress.